Bio21 Institute, School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia.
Cesar Australia, Brunswick, Victoria, Australia.
Environ Microbiol. 2023 Oct;25(10):1988-2001. doi: 10.1111/1462-2920.16432. Epub 2023 Jun 7.
There is increasing interest in the use of endosymbionts in pest control, which will benefit from the identification of endosymbionts from potential donor species for transfer to pest species. Here, we screened for endosymbionts in 123 Australian aphid samples across 32 species using 16S DNA metabarcoding. We then developed a qPCR method to validate the metabarcoding data set and to monitor endosymbiont persistence in aphid cultures. Pea aphids (Acyrthosiphon pisum) were frequently coinfected with Rickettsiella and Serratia, and glasshouse potato aphids (Aulacorthum solani) were coinfected with Regiella and Spiroplasma; other secondary endosymbionts detected in samples occurred by themselves. Hamiltonella, Rickettsia and Wolbachia were restricted to a single aphid species, whereas Regiella was found in multiple species. Rickettsiella, Hamiltonella and Serratia were stably maintained in laboratory cultures, although others were lost rapidly. The overall incidence of secondary endosymbionts in Australian samples tended to be lower than recorded from aphids overseas. These results indicate that aphid endosymbionts probably exhibit different levels of infectivity and vertical transmission efficiency across hosts, which may contribute to natural infection patterns. The rapid loss of some endosymbionts in cultures raises questions about factors that maintain them under field conditions, while endosymbionts that persisted in laboratory culture provide candidates for interspecific transfers.
人们对利用内共生体进行害虫防治越来越感兴趣，这将得益于鉴定潜在供体物种中的内共生体，并将其转移到害虫物种中。在这里，我们使用 16S DNA 代谢组学对来自 32 个物种的 123 个澳大利亚蚜虫样本进行了内共生体筛选。然后，我们开发了一种 qPCR 方法来验证代谢组学数据集，并监测蚜虫培养物中内共生体的持久性。豌豆蚜（Acyrthosiphon pisum）经常与 Rickettsiella 和 Serratia 共感染，而温室马铃薯蚜（Aulacorthum solani）与 Regiella 和 Spiroplasma 共感染；在样本中检测到的其他次要共生体则单独存在。Hamiltonella、Rickettsia 和 Wolbachia 仅局限于一种蚜虫，而 Regiella 则存在于多种物种中。尽管其他共生体迅速丢失，但 Rickettsiella、Hamiltonella 和 Serratia 在实验室培养中得以稳定维持。澳大利亚样本中次要内共生体的总体发生率似乎低于海外蚜虫的记录。这些结果表明，蚜虫内共生体在不同宿主中的感染性和垂直传播效率可能存在不同水平，这可能有助于解释其自然感染模式。一些共生体在培养物中的迅速丢失引发了关于在野外条件下维持它们的因素的问题，而在实验室培养中持续存在的共生体则为种间转移提供了候选者。
