Pest and Environmental Adaptation Research Group, School of biosciences, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Australia.
Section for Bioscience and Engineering, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Environ Microbiol. 2024 Oct;26(10):e16704. doi: 10.1111/1462-2920.16704.
There is increasing interest in exploring how endosymbionts could be useful in pest control, including in aphids, which can carry a diversity of endosymbionts. Endosymbionts often have a large impact on host traits, and their presence can be self-sustaining. Identifying useful host-endosymbiont combinations for pest control is facilitated by the transfer of specific endosymbionts into target species, particularly if the species lacks the endosymbiont. Here, we complete a comprehensive literature review, which included 56 relevant papers on endosymbiont transfer experiments in aphids, to uncover factors that might influence transfer success. We then report on our own microinjection attempts of diverse facultative endosymbionts from a range of donor species into three agriculturally important aphid species as recipients: the green peach aphid (Myzus persicae), bird cherry-oat aphid (Rhopalosiphum padi), and Russian wheat aphid (Diuraphis noxia). Combining this information, we consider reasons that impact the successful establishment of lines carrying transferred endosymbionts. These include a lack of stability in donors, deleterious effects on host fitness, the absence of plant-based (versus vertical) transmission, high genetic variation in the endosymbiont, and susceptibility of an infection to environmental factors. Taking these factors into account should help in increasing success rates in future introductions.
人们越来越关注探索内共生体如何在害虫防治中发挥作用，包括在蚜虫中，蚜虫可以携带多种内共生体。内共生体通常对宿主特征有很大的影响，它们的存在可以自我维持。通过将特定的内共生体转移到目标物种中，可以识别出有用的宿主-内共生体组合来防治害虫，特别是如果该物种缺乏内共生体的话。在这里，我们完成了一项全面的文献综述，其中包括 56 篇关于蚜虫内共生体转移实验的相关论文，以揭示可能影响转移成功的因素。然后，我们报告了我们自己的微注射尝试，将来自多种供体物种的多种兼性内共生体注入三种农业上重要的蚜虫物种作为受体：桃蚜（Myzus persicae）、禾谷缢管蚜（Rhopalosiphum padi）和禾谷长管蚜（Diuraphis noxia）。结合这些信息，我们考虑了影响携带转移内共生体的系成功建立的原因。这些原因包括供体缺乏稳定性、对宿主适应性的有害影响、缺乏基于植物的（而非垂直的）传播、内共生体的遗传高度变异以及感染对环境因素的敏感性。考虑到这些因素应该有助于提高未来引入的成功率。
