Department of Molecular Biology and Genetics, Faculty of Science and Art, Inonu University , Malatya, Turkey.
Arch Microbiol. 2023 Jun 8;205(7):258. doi: 10.1007/s00203-023-03599-5.
Probiotic microorganisms are increasing their interest today due to the benefits they provide to humans. Vinegar is the process of processing foods containing carbohydrates that can be fermented by acetic acid bacteria and yeasts. Hawthorn vinegar is also important in terms of amino acids, aromatic compounds, organic acids, vitamins and minerals it contains. Depending on the variety of microorganisms in it, the content of hawthorn vinegar changes, especially its biological activity. Bacteria were isolated from handmade hawthorn vinegar obtained in this study. After performing its genotypic characterization, it has been tested that it can grow in low pH environment, survive in artificial gastric and small intestinal fluid, survive against bile acids, surface adhesion characteristics, antibiotic susceptibility, adhesion, and degrade various cholesterol precursors. According to the results obtained, the studied isolate is Levilactobacillus brevis, it can reproduce best at pH 6.3, survives 72.22% in simulated gastric juice, 69.59% in small intestinal fluid, and 97% adhesion to HTC-116. Partially reproduces even in the presence of 2% ox-bile, surface hydrophobicity is 46.29% for n-hexadecane. It has been determined that it can degrade 4 different cholesterol precursors except for Sodium thioglycolate and is generally resistant to antibiotics except for CN30 and N30. Considering the experimental findings of Levilactobacillus brevis isolated from hawthorn vinegar for the first time, it can be said that Levilactobacillus brevis has probiotic properties.
由于益生菌微生物为人类带来的益处，它们如今越来越受到关注。醋是一种加工含有碳水化合物的食物的过程，这些碳水化合物可以被醋酸菌和酵母发酵。山楂醋在氨基酸、芳香化合物、有机酸、维生素和矿物质方面也很重要。根据其中微生物的种类不同，山楂醋的含量会发生变化，尤其是其生物活性。本研究从手工山楂醋中分离出细菌。对其进行基因特征描述后，发现其能够在低 pH 环境中生长，在人工胃液和肠液中存活，能够抵抗胆盐，具有表面黏附特性、抗生素敏感性、黏附和降解各种胆固醇前体的能力。根据所得结果，研究分离出的细菌是短乳杆菌，其在 pH6.3 时生长最佳，在模拟胃液中存活率为 72.22%，在肠液中存活率为 69.59%，对 HTC-116 的黏附率为 97%。即使在存在 2%牛胆盐的情况下，也能部分繁殖，对正十六烷的表面疏水性为 46.29%。结果表明，除了巯基乙酸钠外，它可以降解 4 种不同的胆固醇前体，除了对 CN30 和 N30 外，通常对抗生素具有抗性。考虑到首次从山楂醋中分离出的短乳杆菌的实验发现，可以说短乳杆菌具有益生菌特性。
