Worku Solomon Ayenew, Tadesse Solomon Asmamaw, Abdelwuhab Mohammedbrhan, Asrie Assefa Belay
Hospital Pharmacy, University of Gondar, Gondar, Ethiopia.
Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Heliyon. 2023 May 25;9(6):e16386. doi: 10.1016/j.heliyon.2023.e16386. eCollection 2023 Jun.
In Ethiopian traditional medicine, is one of the most often utilized medicinal herbs for the treatment of diarrhea. Therefore, this study was conducted to validate the use of the plant for the treatment of diarrhea in the traditional medical practice of Ethiopia.
Castor oil-induced diarrhea, enteropooling, and intestinal motility test models in mice were used to evaluate the antidiarrheal properties of the 80% methanol crude extract and the solvent fractions of the root component of . The effects of the crude extract and the fractions on time for onset, frequency, weight, and water content of diarrheal feces, intestinal fluid accumulation, and intestinal transit of charcoal meal were evaluated and compared with the corresponding results in the negative control.
The crude extract (CE), aqueous fraction (AQF), and ethyl acetate fraction (EAF) at 400 mg/kg ( < 0.001) significantly delayed the onset of diarrhea. Besides, the CE and AQF at 200 and 400 mg/kg (p < 0.001) of the doses, and EAF at 200 (p < 0.01) and 400 mg/kg (p < 0.001) significantly decreased the frequency of diarrheal stools. Furthermore, CE, AQF, and EAF at their three serial doses (p < 0.001), significantly reduced the weights of the fresh diarrheal stools as compared to the negative control. The CE and AQF at 100 (p < 0.01), and 200 and 400 mg/kg (p < 0.001) of their doses and EAF at 200 (p < 0.01) and 400 mg/kg (p < 0.001) significantly decreased the fluid contents of diarrheal stools compared to the negative control. In the enteropooling test, the CE at 100 (p < 0.05), and 200 and 400 mg/kg (p < 0.001), AQF at 200 (P < 0.05) and 400 mg/kg (p < 0.01), and EAF at 200 (p < 0.01) and 400 mg/kg (p < 0.001) significantly decreased the weights of intestinal contents compared to the negative control. Additionally, the CE at 100 and 200 (p < 0.05) and 400 mg/kg (p < 0.001), AQF at 100 (p < 0.05), 200 (p < 0.01), and 400 mg/kg (p < 0.001) of the doses, and EAF at 400 mg/kg (p < 0.05), produced significant reductions in the volumes of intestinal contents. In the intestinal motility test model, the CE, AQF, and EAF at all their serial doses (p < 0.001), significantly suppressed the intestinal transit of charcoal meal and peristaltic index compared to the negative control.
Overall, the results of this study showed that the crude extract and the solvent fractions of the root parts of had considerable antidiarrheal activities. Besides, the crude extract, especially at 400 mg/kg, produced the highest effect followed by the aqueous fraction at the same dose. This might indicate that the bioactive compounds responsible for the effects are more of hydrophilic in nature. Moreover, the antidiarrheal index values were increased with the doses of the extract and the fractions, suggesting that the treatments might have dose-dependent antidiarrheal effects. Additionally, the extract was shown to be free of observable acute toxic effects. Thus, this study corroborates the use the root parts of to treat diarrhea in the traditional settings. Furthermore, the findings of this study are encouraging and may be used as the basis to conduct further studies in the area including chemical characterization and molecular based mechanism of actions of the plant for its confirmed antidiarrheal effects.
在埃塞俄比亚传统医学中，[植物名称未给出]是治疗腹泻最常用的草药之一。因此，本研究旨在验证该植物在埃塞俄比亚传统医学实践中治疗腹泻的用途。
采用蓖麻油诱导的小鼠腹泻、肠积液和肠道运动试验模型，评估[植物名称未给出]根成分的80%甲醇粗提物及其溶剂提取物的止泻特性。评估粗提物和提取物对腹泻粪便的起效时间、频率、重量和含水量、肠液积聚以及炭末肠道转运的影响，并与阴性对照的相应结果进行比较。
400mg/kg的粗提物（CE）、水相提取物（AQF）和乙酸乙酯提取物（EAF）（p<0.001）显著延迟腹泻的发作。此外，200和400mg/kg剂量的CE和AQF（p<0.001），以及200（p<0.01）和400mg/kg（p<0.001）的EAF显著降低腹泻粪便的频率。此外，与阴性对照相比，CE、AQF和EAF在三个连续剂量下（p<0.001）显著降低了新鲜腹泻粪便的重量。100（p<0.01）、200和400mg/kg剂量的CE和AQF以及200（p<0.01）和400mg/kg（p<0.001）的EAF与阴性对照相比，显著降低了腹泻粪便的液体含量。在肠积液试验中，100（p<0.05）、200和400mg/kg的CE（p<0.001），200（P<0.05）和400mg/kg的AQF（p<0.01），以及200（p<0.01）和400mg/kg的EAF（p<0.001）与阴性对照相比，显著降低了肠内容物的重量。此外，100和200（p<0.05）以及400mg/kg（p<0.001）的CE，100（p<0.05）、200（p<0.01）和400mg/kg（p<0.001）的AQF，以及400mg/kg（p<0.05）的EAF显著降低了肠内容物的体积。在肠道运动试验模型中，与阴性对照相比，CE、AQF和EAF在所有连续剂量下（p<0.001）均显著抑制了炭末的肠道转运和蠕动指数。
总体而言，本研究结果表明，[植物名称未给出]根的粗提物和溶剂提取物具有相当的止泻活性。此外，粗提物，尤其是400mg/kg时，产生的效果最高，其次是相同剂量的水相提取物。这可能表明起作用的生物活性化合物在性质上更具亲水性。此外，止泻指数值随提取物和提取物剂量的增加而增加，表明这些治疗可能具有剂量依赖性的止泻作用。此外，提取物未显示出明显的急性毒性作用。因此，本研究证实了在传统环境中使用[植物名称未给出]根治疗腹泻的用途。此外，本研究的结果令人鼓舞，可作为在该领域进行进一步研究的基础，包括对该植物已证实的止泻作用进行化学表征和基于分子的作用机制研究。
