Andargie Yared, Sisay Woretaw, Molla Mulugeta, Adela Muluken
School of Pharmacy, College of Medicine and Health Science, Debre Tabor University, Debre Tabor, Ethiopia.
Metabol Open. 2022 Jun 20;15:100197. doi: 10.1016/j.metop.2022.100197. eCollection 2022 Sep.
Natural products have been utilized by human beings for thousands of years to relieve a variety of ailments, including diarrhea. Conventional antidiarrheal drugs are associated with multiple adverse effects and contraindications. Traditionally, crushing the root, mix with water and then drunk the juice is extensively used for treating diarrhea. However, no scientific research has been done yet to support its antidiarrheal efficacy and safety. Hence, the aim of the study was to evaluate the antidiarrheal activity and safety profile of the plant in mice.
The hydro-methanolic extract was extracted through a cold maceration technique using 80% methanol. Castor oil-induced diarrheal, gastro-intestinal transit, and enteropooling models have been employed to assess the antidiarrheal activity of the test extract at doses of 100, 200, and 400 mg/kg.
The crude root extract caused no mortality at a single limit test dose of 2 g/kg throughout the first 24 h and for the rest of the 14 days. In a castor oil-induced diarrheal model, the hydro-methanolic extract markedly delayed the onset of diarrhea, reduced the weight of wet and total feces at 100 (<.05), 200 (<.01), and 400 mg/kg (<.001) test doses. Meanwhile, at 200 (<.01) and 400 mg/kg (<.001) doses, the plant extract considerably lowered the weight and volume of intestinal contents. In the gastro-intestinal transit model, however, a dramatic inhibition in the charcoal meal travel was noticed at 100 (<.05), 200 (<.01), and 400 mg/kg (<.001) test doses. The peak antidiarrheal index was exhibited at the highest dose of the test extract.
The study speculated that root extract possesses antidiarrheal activity, which could be owing to its inhibitory effect on both gastro-intestinal motility and fluid secretion.
数千年来，天然产物一直被人类用于缓解包括腹泻在内的各种疾病。传统止泻药存在多种不良反应和禁忌证。传统上，将该植物的根碾碎，与水混合后饮用汁液被广泛用于治疗腹泻。然而，尚未有科学研究支持其止泻功效和安全性。因此，本研究的目的是评估该植物在小鼠体内的止泻活性和安全性。
采用冷浸法，用80%甲醇提取水甲醇提取物。采用蓖麻油诱导的腹泻、胃肠转运和肠积液模型，评估受试提取物在100、200和400mg/kg剂量下的止泻活性。
在单次极限试验剂量2g/kg下，粗根提取物在最初24小时及随后14天内均未导致死亡。在蓖麻油诱导的腹泻模型中，水甲醇提取物在100（<.05）、200（<.01）和400mg/kg（<.001）试验剂量下显著延迟腹泻发作，减轻湿粪和总粪便重量。同时，在200（<.01）和400mg/kg（<.001）剂量下，植物提取物显著降低肠内容物的重量和体积。然而，在胃肠转运模型中，在100（<.05）、200（<.01）和400mg/kg（<.001）试验剂量下，观察到炭末推进有显著抑制作用。受试提取物最高剂量时止泻指数达到峰值。
本研究推测该植物根提取物具有止泻活性，这可能归因于其对胃肠动力和液体分泌的抑制作用。
