Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.
Department of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Front Immunol. 2023 May 23;14:1163350. doi: 10.3389/fimmu.2023.1163350. eCollection 2023.
Myocardial infarction (MI) is a common cardiac condition with a high incidence of morbidity and mortality. Despite extensive medical treatment for MI, the development and outcomes of post-MI heart failure (HF) continue to be major factors contributing to poor post-MI prognosis. Currently, there are few predictors of post-MI heart failure.
In this study, we re-examined single-cell RNA sequencing and bulk RNA sequencing datasets derived from the peripheral blood samples of patients with myocardial infarction, including patients who developed heart failure and those who did not develop heart failure after myocardial infarction. Using marker genes of the relevant cell subtypes, a signature was generated and validated using relevant bulk datasets and human blood samples.
We identified a subtype of immune-activated B cells that distinguished post-MI HF patients from non-HF patients. Polymerase chain reaction was used to confirm these findings in independent cohorts. By combining the specific marker genes of B cell subtypes, we developed a prediction model of 13 markers that can predict the risk of HF in patients after myocardial infarction, providing new ideas and tools for clinical diagnosis and treatment.
Sub-cluster B cells may play a significant role in post-MI HF. We found that the , and genes in patients with post-MI HF showed the same trend of increase as those without post-MI HF.
心肌梗死（MI）是一种常见的心脏疾病，发病率和死亡率都很高。尽管对 MI 进行了广泛的医疗治疗，但 MI 后心力衰竭（HF）的发展和结局仍然是导致预后不良的主要因素。目前，MI 后心力衰竭的预测因素很少。
在这项研究中，我们重新检查了来自心肌梗死患者外周血样本的单细胞 RNA 测序和批量 RNA 测序数据集，包括发生心力衰竭和未发生心力衰竭的患者。使用相关细胞亚型的标记基因，生成一个签名，并使用相关的批量数据集和人类血液样本进行验证。
我们确定了一种免疫激活 B 细胞亚型，可以将 MI 后 HF 患者与非 HF 患者区分开来。聚合酶链反应用于在独立队列中证实这些发现。通过结合 B 细胞亚型的特定标记基因，我们开发了一个由 13 个标记物组成的预测模型，可以预测心肌梗死后患者发生 HF 的风险，为临床诊断和治疗提供了新的思路和工具。
亚群 B 细胞可能在 MI 后 HF 中起重要作用。我们发现，MI 后 HF 患者的 和 基因的表达趋势与无 MI 后 HF 患者相同。
