Li Xin, Sun Yanan, Wang Xiaochen
National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Biophys Rep. 2021 Feb 28;7(1):1-7. doi: 10.52601/bpr.2021.200047.
Lysosomes are membrane-bound organelles for biomolecule degradation and recycling. They also serve as a nutrient sensing and signaling center to maintain cell and tissue homeostasis. Lysosomal properties alter in response to developmental or environmental cues, but these changes are hard to track . Employing as a model system, we have developed assays to examine and quantify lysosome properties , including lysosome maturation, acidification and cleavage activity. These assays can be used to reveal alterations of lysosomal activity during development and in stress conditions.
溶酶体是用于生物分子降解和循环利用的膜结合细胞器。它们还作为营养感知和信号传导中心，以维持细胞和组织的稳态。溶酶体的特性会根据发育或环境线索而改变，但这些变化很难追踪。以 作为模型系统，我们开发了检测方法来检查和量化溶酶体特性，包括溶酶体成熟、酸化和裂解活性。这些检测方法可用于揭示发育过程中和应激条件下溶酶体活性的改变。
