Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
School of Medicine, National Tsing Hua University, Hsinchu, Taiwan.
Oral Dis. 2024 Apr;30(3):1483-1487. doi: 10.1111/odi.14638. Epub 2023 Jun 8.
Periodontitis is an inflammatory disease caused by bacteria present in the dental biofilm. However, the presence of two oral protozoans, Entamoeba gingivalis and Trichomonas tenax, in patients with the periodontal disease remains largely unknown in Taiwan. Therefore, we investigated the prevalence of oral microbial infections between the sites with mild gingivitis and chronic periodontitis in patients.
We collected 60 dental biofilm samples from sites with mild gingivitis (probing depth <5 mm) and chronic periodontitis (probing depth ≥5 mm) from 30 patients at the National Cheng Kung University Hospital. The samples were analyzed via polymerase chain reaction and gel electrophoresis.
Among oral protozoans, E. gingivalis and T. tenax were detected in 44 (74.07%) and 14 (23.33%) of all samples, respectively. Among oral bacteria, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia were detected in 50 (83.33%), 47 (78.33%), and 48 (80.0%) samples, respectively.
This study, which is the first to analyze E. gingivalis and T. tenax presence among patients with periodontitis in Taiwan, revealed an association between periodontitis and oral microbes.
牙周炎是一种由存在于牙菌斑中的细菌引起的炎症性疾病。然而，在台湾，牙周病患者口腔中两种原生动物，即牙龈内阿米巴（Entamoeba gingivalis）和阴道毛滴虫（Trichomonas tenax）的存在情况在很大程度上仍不清楚。因此，我们调查了患有轻度牙龈炎和慢性牙周炎的患者之间口腔微生物感染的患病率。
我们从国立成功大学医院的 30 名患者的轻度牙龈炎（探诊深度 <5 毫米）和慢性牙周炎（探诊深度 ≥5 毫米）部位收集了 60 个牙菌斑样本。通过聚合酶链反应和凝胶电泳对样本进行分析。
在口腔原生动物中，分别在所有样本的 44 个（74.07%）和 14 个（23.33%）中检测到牙龈内阿米巴和阴道毛滴虫。在口腔细菌中，分别在 50 个（83.33%）、47 个（78.33%）和 48 个（80.0%）样本中检测到牙龈卟啉单胞菌、密螺旋体和福赛斯坦纳菌。
这项研究首次分析了台湾牙周病患者中牙龈内阿米巴和阴道毛滴虫的存在情况，揭示了牙周炎与口腔微生物之间的关联。
