Department of Oral Medicine Dental Section.
Department of Orthopaedic Surgery.
J Ayub Med Coll Abbottabad. 2023 Oct-Dec;35(Suppl 1)(4):S732-S739. doi: 10.55519/JAMC-S4-12660.
A group of eukaryotic organisms that are either free-living or parasitic and feed on organic matter, such as microorganisms or organic detritus. This group is referred to as a polyphyletic classification. They can be found in a wide variety of patterns and sizes, ranging from an amoeba, which can vary its shape, to a paramecium, which has a fixed shape and a complicated structure. The objective was to find out the occurrence of Entamoeba gingivalis and Trichomonas among Dental Patients visiting the Periodontology Department Dental Section, Sandeman, Provincial Hospital Quetta.
The study design was randomized cross-sectional and conducted in the Department of Periodontology Dental Section, Sandeman Provincial Hospital Quetta, from March 2022 to February 2023. A total of 110 known cases of periodontitis and gingivitis were recruited in this study, and further to laboratory work the bacterial biofilm samples were collected from both gingivitis and periodontitis patients. The dental plaque was placed on individual glass microscope slides and a drop of saline and mixed and covered with a coverslip. The wet smear was examined immediately under 40X, and the objective for the presence and absence of motile amoebae or flagellated protozoa was recorded. .
out of 110 known cases of periodontitis and gingivitis 91 (83%) patients were males and 19 (17%) were females among 110 patients, 40 (36%) patients were periodontitis and 70 (64%) patients were gingivitis. Among 40 (36%) periodontitis patients 3(7.5%) were Entamoeba gingivalis and 4(10%) were Trichomonas Tenax while 70 (64%) cases of Gingivitis 20 (28%) of Entamoeba gingivalis and 1(1%) Trichomonas tenax was recorded. No patient had both species of protozoa were seen in this study.
The results of this study revealed the frequency of occurrence of E. gingivalis and T. tenax and the state of periodontitis and gingivitis. The prevalence of E. gingivalis was higher than T. tenax. We recommended further research with a higher number of patients and may use more advanced and reliable laboratory techniques such as PCR and electron microscopy.
一组真核生物，它们要么是自由生活的，要么是寄生的，以有机物为食，如微生物或有机碎屑。这一组被称为多系分类。它们的形态和大小多种多样，从可以改变形状的变形虫到具有固定形状和复杂结构的草履虫。目的是了解在桑德曼省级医院牙周科就诊的牙周病和牙龈炎患者中是否存在牙龈内阿米巴和滴虫。
本研究设计为随机横断面研究，于 2022 年 3 月至 2023 年 2 月在桑德曼省级医院牙周科进行。本研究共招募了 110 名已知的牙周炎和牙龈炎患者，进一步进行实验室工作，从牙龈炎和牙周炎患者中采集细菌生物膜样本。将牙菌斑放在单独的载玻片上，滴一滴生理盐水并混合，盖上盖玻片。立即在 40X 下检查湿抹片，并记录是否存在游动的变形虫或鞭毛原生动物。
在 110 名已知的牙周炎和牙龈炎患者中，91 名（83%）为男性，19 名（17%）为女性，110 名患者中，40 名（36%）为牙周炎患者，70 名（64%）为牙龈炎患者。在 40 名（36%）牙周炎患者中，有 3 名（7.5%）为牙龈内阿米巴，4 名（10%）为滴虫 Tenax，而在 70 名（64%）牙龈炎患者中，有 20 名（28%）为牙龈内阿米巴，1 名（1%）为滴虫 Tenax。在这项研究中，没有患者同时存在这两种原生动物。
本研究结果揭示了 E. gingivalis 和 T. tenax 的发生频率以及牙周炎和牙龈炎的状态。E. gingivalis 的患病率高于 T. tenax。我们建议进一步研究更多的患者，并可能使用更先进和可靠的实验室技术，如 PCR 和电子显微镜。
