Department of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Tropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Parasitology. 2023 Jul;150(8):653-660. doi: 10.1017/S0031182023000112. Epub 2023 Feb 17.
is a carcinogenic parasite that can cause bile duct cancer called cholangiocarcinoma. A study of the immune response of this parasite in susceptible and non-susceptible hosts may provide a clue to develop vaccines and immunodiagnostic markers, which are currently not available. Here, we compared the antibody response in susceptible Golden Syrian hamsters and non-susceptible BALB/c mice infected by the liver fluke. In mice, the antibody was detected between 1 and 2 weeks post-infection, whereas it was positive between 2 and 4 weeks post-infection in hamsters. Immunolocalization revealed that the antibody from mice reacts strongly with the tegumental surface and gut epithelium of the worm, while hamster antibody showed a weak signal in the tegument and a comparable signal in the gut of the worm. Immunoblot of the tegumental proteins demonstrated that while hamster antibody showed a broad specificity, mice strongly reacted with a single protein band. Mass spectrometry revealed these immunogenic targets. Recombinant proteins of the reactive targets were produced in the bacterial expression system. The immunoblot of these recombinant proteins confirm the reactivity of their native form. In summary, there is a different antibody response against infection in susceptible and non-susceptible hosts. The non-susceptible host reacts quicker and stronger than the susceptible host.
华支睾吸虫是一种致癌寄生虫，可引起胆管癌，称为胆管癌。研究这种寄生虫在易感和不易感宿主中的免疫反应可能为开发疫苗和免疫诊断标志物提供线索，而目前这些疫苗和免疫诊断标志物尚不可用。在这里，我们比较了易感金黄地鼠和不易感 BALB/c 小鼠感染肝吸虫后的抗体反应。在小鼠中，抗体在感染后 1 至 2 周内检测到，而在感染后 2 至 4 周内，在金黄地鼠中检测到抗体。免疫定位显示，来自小鼠的抗体与虫体的表皮和肠道上皮强烈反应，而仓鼠抗体在表皮上的信号较弱，在虫体的肠道上的信号相当。表皮蛋白的免疫印迹显示，尽管仓鼠抗体表现出广泛的特异性，但小鼠强烈反应于单一的蛋白质带。质谱揭示了这些免疫原性靶标。反应性靶标的重组蛋白在细菌表达系统中产生。这些重组蛋白的免疫印迹证实了其天然形式的反应性。总之，在易感和不易感宿主中，针对 感染存在不同的抗体反应。不易感宿主的反应比易感宿主更快、更强。
