Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi, India.
Department of Pediatrics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Pediatr Hematol Oncol. 2023;40(6):568-586. doi: 10.1080/08880018.2023.2218409. Epub 2023 Jun 8.
Low birth weight (LBW) is a leading cause of newborn's mortality however the underlying defects in cellular immunity and immune mechanisms leading to severe neonatal infections in term LBW (tLBW) newborns are not well understood. Neutrophil extracellular traps (NETs), or NETosis, is an innate immune defense mechanism of neutrophils involved in trapping and killing of microbes. The efficiency of NET formation in cord blood derived neutrophils of tLBW and normal birth weight (NBW) newborns in the presence of toll like receptor (TLR) agonist inductions was evaluated. The NET formation was observed to be substantially impaired in tLBW newborns along with NET proteins expression, extracellular deoxyribonucleic acid (DNA) release and reactive oxygen species generation. The placental tissues from tLBW newborns delivery also showed minimal NETosis. These findings suggest impaired NET formation to be an important factor underlying the deficient immune status of tLBW newborns making them susceptible to life- threatening infections.
低出生体重（LBW）是新生儿死亡的主要原因，但导致足月 LBW（tLBW）新生儿严重新生儿感染的细胞免疫和免疫机制的潜在缺陷尚不清楚。中性粒细胞胞外诱捕网（NETs）或 NETosis 是中性粒细胞参与捕获和杀死微生物的固有免疫防御机制。评估了 TLR 激动剂诱导下 tLBW 和正常出生体重（NBW）新生儿脐带血来源中性粒细胞中 NET 形成的效率。结果发现，tLBW 新生儿的 NET 形成明显受损，同时 NET 蛋白表达、细胞外脱氧核糖核酸（DNA）释放和活性氧生成减少。来自 tLBW 新生儿分娩的胎盘组织也显示出最小的 NETosis。这些发现表明，NET 形成受损是 tLBW 新生儿免疫状态缺陷的一个重要因素，使他们容易受到危及生命的感染。
