Department of Biochemistry & Medical Biotechnology, School of Tropical Medicine, Kolkata, 700073, India.
Future Microbiol. 2023 May;18:489-503. doi: 10.2217/fmb-2022-0240. Epub 2023 Jun 8.
In this study, uropathogenic (UPEC) from pregnant and nonpregnant patients were characterized with respect to antimicrobial resistance (AMR) and expression of their virulence factors and cytokines elicited upon infection in urothelial (HTB-4) cells to frame proper therapeutics. Antibiotic sensitivity and adherence to HTB-4 cells were tested and PCR and real-time PCR were performed. UPEC from nonpregnant patients showed the most resistance with a significant correlation between the expression of and and and . The expression of and , and , and and , respectively, were significantly correlated in UPEC from pregnant patients. Cytokine expression profiles were correlated with the expression of virulence genes in UPEC that was isolated from different populations, and should be accounted for along with AMR analysis.
在这项研究中，对来自孕妇和非孕妇患者的尿路致病性大肠杆菌（UPEC）进行了特征描述，包括其对感染尿路上皮（HTB-4）细胞的抗生素耐药性（AMR）和毒力因子表达的研究，以制定合适的治疗方案。检测了抗生素敏感性和对 HTB-4 细胞的黏附性，并进行了 PCR 和实时 PCR 实验。非孕妇患者的 UPEC 表现出最强的耐药性，并且与 、 和 之间的表达存在显著相关性。孕妇患者的 UPEC 中， 、 、 和 、 、 之间的表达分别呈显著相关性。来自不同人群的 UPEC 的细胞因子表达谱与毒力基因的表达相关，这应该与 AMR 分析一起考虑。
