人类糖尿病微血管病变中肾小球基底膜的生化研究。

Biochemical studies on glomerular basement membrane in human diabetic microangiopathy.

作者信息

Cruz A, Moreau Lalande H

出版信息

Pathol Biol (Paris). 1978 Oct;26(7):411-7.

Abstract

Biochemical modifications of glomerular basement membrane (GBM) in diabetes mellitus have been investigated in human subjects. Amino acid analysis and electrophoretic study of unfractioned GBM from diabetic and non diabetic kidneys showed an increase in hydroxylysine, hydroxyproline and glycine and in the glycosylgalactosyl-hydroxylysine (G-G-H) content of diabetic GBM. A decrease in 1/2 cystine was also demonstrated in diabetes. Fractionation of GBM showed the increase in G-G-H observed on unfractioned GBM to reflect a quantitative increase of the "collagen" fraction in diabetes, the amino acid composition of this fraction remaining unchanged. The decrease in 1/2 cystine demonstrated on unfractioned GBM was located in the non collagenous fractions of this structure.

摘要

在人类受试者中，已对糖尿病患者肾小球基底膜（GBM）的生化修饰进行了研究。对糖尿病和非糖尿病肾脏中未分级的GBM进行氨基酸分析和电泳研究表明，糖尿病GBM中的羟赖氨酸、羟脯氨酸、甘氨酸以及糖基半乳糖基羟赖氨酸（G-G-H）含量增加。糖尿病患者中还证实了半胱氨酸含量减少。GBM分级显示，未分级GBM中观察到的G-G-H增加反映了糖尿病中“胶原蛋白”部分的定量增加，该部分的氨基酸组成保持不变。未分级GBM中显示的半胱氨酸减少位于该结构的非胶原部分。