Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea.
Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea; Korean Medicine Data Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
J Hazard Mater. 2023 Sep 5;457:131761. doi: 10.1016/j.jhazmat.2023.131761. Epub 2023 Jun 2.
Concerns about antibiotic resistance genes (ARGs) released from wastewaters of livestock or fish farming into the natural environment are increasing, but studies on unculturable bacteria related to the dissemination of antibiotic resistance are limited. Here, we reconstructed 1100 metagenome-assembled genomes (MAGs) to assess the impact of microbial antibiotic resistome and mobilome in wastewaters discharged to Korean rivers. Our results indicate that ARGs harbored in the MAGs were disseminated from wastewater effluents into downstream rivers. Moreover, it was found that ARGs are more commonly co-localized with mobile genetic elements (MGEs) in agricultural wastewater than in river water. Among the effluent-derived phyla, uncultured members of the superphylum Patescibacteria possessed a high number of MGEs, along with co-localized ARGs. Our findings suggest that members of the Patesibacteria are a potential vector for propagating ARGs into the environmental community. Therefore, we propose that the dissemination of ARGs by uncultured bacteria should be further investigated in multiple environments.
人们越来越关注来自畜牧或水产养殖废水的抗生素耐药基因 (ARGs) 释放到自然环境中所带来的问题，但有关与抗生素耐药性传播相关的不可培养细菌的研究还很有限。在这里，我们重建了 1100 个宏基因组组装基因组 (MAG)，以评估微生物抗生素耐药组和移动组在排放到韩国河流中的废水中的影响。我们的研究结果表明，MAGs 中携带的 ARGs 从废水处理厂排放到下游河流中。此外，还发现 ARGs 更常见于农业废水中与移动遗传元件 (MGE) 共定位，而不是在河水中。在废水衍生的门中，未培养的 Patescibacteria 超门成员拥有大量的 MGEs，以及共定位的 ARGs。我们的研究结果表明，Patesibacteria 成员可能是将 ARGs 传播到环境群落中的潜在载体。因此，我们建议在多个环境中进一步研究不可培养细菌传播 ARGs 的情况。
