State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Ningxia Eppen Biotech Co., Ltd., China Agricultural University, Beijing 100193, China.
Bioresour Technol. 2023 Sep;384:129278. doi: 10.1016/j.biortech.2023.129278. Epub 2023 Jun 7.
In this study, α-L-arabinofuranosidase (AF) from Aspergillus awamori was heterologously expressed in Pichia pastoris X33, with a 1-fold increase in AF activity after codon and vector optimization. AF remained stable at 60-65 °C and displayed a broad pH stability range of 2.5-8.0. It also demonstrated considerable resistance to pepsin and trypsin. Furthermore, compared with xylanase alone, AF with xylanase exhibited a marked synergistic effect in the degradation of expanded corn bran, corn bran, and corn distillers' dried grains with solubles, reducing sugars by 3.6-fold, 1.4-fold, and 6.5-fold, respectively, with the degree of synergy increasing to 4.61, 2.44, and 5.4, respectively, while in vitro dry matter digestibility values were 17.6%, 5.2%, and 8.8%, respectively. After enzymatic saccharification, corn byproducts were converted to prebiotic xylo-oligosaccharides and arabinoses, thereby demonstrating the favorable properties of AF in the degradation of corn biomass and its byproducts.
在这项研究中，米曲霉 α-L-阿拉伯呋喃糖苷酶（AF）通过密码子和载体优化在毕赤酵母 X33 中进行了异源表达，酶活提高了 1 倍。AF 在 60-65°C 下保持稳定，显示出 2.5-8.0 的宽 pH 稳定性范围。它还对胃蛋白酶和胰蛋白酶具有相当的抗性。此外，与单独使用木聚糖酶相比，AF 与木聚糖酶在降解膨化玉米麸皮、玉米麸皮和玉米酒糟可溶物方面表现出显著的协同作用，还原糖分别提高了 3.6 倍、1.4 倍和 6.5 倍，协同度分别提高到 4.61、2.44 和 5.4，而体外干物质消化率分别为 17.6%、5.2%和 8.8%。酶解后，玉米副产品转化为具有益生元特性的木二糖和阿拉伯糖，从而证明了 AF 在降解玉米生物质及其副产品方面的良好性能。
