State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Bioresour Technol. 2024 Feb;394:130177. doi: 10.1016/j.biortech.2023.130177. Epub 2023 Dec 10.
Utilizing thermostable enzymes in biomass conversion processes presents a promising approach to bypass pretreatment, garnering significant attention from the biorefinery industry. A novel discovered α-l-arabinofuranosidase, Abf4980, exhibits exceptional thermostability by maintaining full activity after 24 h of incubation at 70 °C. It effectively acts on polyarabinosides, cleaving α-1,2- and α-1,3-linked arabinofuranose side chains from water-soluble wheat arabinoxylan while releasing xylose. When synergistically combined with the thermostable bifunctional xylanase/β-glucanase CbXyn10C from Caldicellulosiruptor bescii at an enzyme-activity ratio of 6:1, Abf4980 achieves the highest degradation efficiency for wheat arabinoxylan. Furthermore, Abf4980 and CbXyn10C demonstrated remarkable efficacy in hydrolyzing unmodified wheat bran and corn cob to generate arabinose and xylooligosaccharides. This discovery holds promising opportunities for improving the efficiency of lignocellulosic biomass conversion into fermentable sugars.
利用热稳定性酶进行生物质转化过程是一种有前途的方法，可以绕过预处理，引起了生物炼制行业的极大关注。一种新发现的α-l-阿拉伯呋喃糖苷酶 Abf4980 在 70°C 孵育 24 小时后仍保持全部活性，表现出非凡的热稳定性。它能有效作用于聚阿拉伯糖苷，从水溶性小麦阿拉伯木聚糖中切割出与α-1,2-和α-1,3-键相连的阿拉伯呋喃糖侧链，并释放出木糖。当与来自 Caldicellulosiruptor bescii 的热稳定双功能木聚糖酶/β-葡聚糖酶 CbXyn10C 以 6:1 的酶活性比协同组合时，Abf4980 对小麦阿拉伯木聚糖的降解效率最高。此外，Abf4980 和 CbXyn10C 在水解未改性的小麦麸皮和玉米芯以生成阿拉伯糖和木低聚糖方面表现出显著的功效。这一发现为提高木质纤维素生物质转化为可发酵糖的效率提供了有希望的机会。
