Fernández-Delgado R, Besalduch J, Momparler P, Balaguer H, García-Mora R, Jubert A, Donat J, Colomer J
An Esp Pediatr. 1986 Apr;24(4):221-6.
Authors study growth patterns of granulocytic-macrophagic progenitors in umbilical cord blood of 21 full-term newborns, using collagen gel culture method. Predominantly they obtain pure macrophagic colonies or mixed granulocytic colonies. After 7 days of incubation mean and SD of clusters in 103.22 +/- 75.88, and mean and SD of colonies is 34.26 +/- 23.1. After 14 days clusters falls down until 47.2 +/- 30.48, but colonies goes up to 74.2 +/- 32.78. Lineal regression analysis shows a correlation coefficient of 0.809 between 7 days and 14 days colonies. These results demonstrate the different behaviour between myelopoietic progenitor from umbilical cord blood and same progenitor from adult blood and are probably related to different nature of both progenitors.
作者采用胶原凝胶培养法研究了21例足月新生儿脐带血中粒系-巨噬系祖细胞的生长模式。他们主要获得了纯巨噬细胞集落或混合粒系集落。培养7天后，集簇的平均值和标准差为103.22±75.88，集落的平均值和标准差为34.26±23.1。培养14天后，集簇数量下降至47.2±30.48，但集落数量上升至74.2±32.78。线性回归分析显示，7天和14天集落之间的相关系数为0.809。这些结果表明，脐带血中的骨髓祖细胞与成人血液中的相同祖细胞具有不同的行为，这可能与两种祖细胞的不同性质有关。
