Ueno Y, Koizumi S, Yamagami M, Miura M, Taniguchi N
Exp Hematol. 1981 Aug;9(7):716-22.
Some properties of hemopoietic stem cells (CFUc) in human cord blood and peripheral blood of neonates were compared with those in bone marrow, by the method for in vitro growth of colony-forming cells in semisolid agar. The number of CFUc circulating in cord blood was 93.5 +/- 57.8 (mean +/- 1 SD)/10(5) cells, which was slightly less than that of bone marrow. The number of CFUc in peripheral blood decreased promptly following birth. From cord and neonatal blood, most colonies were monocyte/macrophage colonies (60.0 +/- 4.6%), whereas those from bone marrow were largely granulocyte colonies (88.3 +/- 6.2%). The differences of growth curve, colony size, and turnover state of CFUc between cord blood and bone marrow seemed, in part, to result from the dominancy of monocyte/macrophage colony-forming cells in cord blood. The cell cycling rate of CFUc in cord blood was remarkably lower than that in bone marrow. From these results, it seems clear that CFUc in cord blood differ in some aspects from those in bone marrow. However, the precise physiologic and functional significance of CFUc circulating in cord and neonatal blood remains unknown.
采用半固体琼脂中集落形成细胞体外生长方法，比较了人脐血、新生儿外周血和骨髓中造血干细胞（CFUc）的一些特性。脐血中循环的CFUc数量为93.5±57.8（均值±1标准差）/10⁵个细胞，略少于骨髓中的数量。出生后外周血中的CFUc数量迅速减少。脐血和新生儿血液中，大多数集落为单核细胞/巨噬细胞集落（60.0±4.6%），而骨髓中的集落主要是粒细胞集落（88.3±6.2%）。脐血和骨髓中CFUc的生长曲线、集落大小和更新状态的差异，部分似乎是由于脐血中单核细胞/巨噬细胞集落形成细胞占优势所致。脐血中CFUc的细胞周期率明显低于骨髓中的。从这些结果来看，脐血中的CFUc在某些方面似乎与骨髓中的不同。然而，脐血和新生儿血液中循环的CFUc的确切生理和功能意义仍不清楚。
