School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.
Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine, Tianjin, 301617, China.
Chem Biodivers. 2023 Jul;20(7):e202300458. doi: 10.1002/cbdv.202300458. Epub 2023 Jun 21.
Polygonati Rhizoma has been a famous traditional Chinese medicine (TCM) for two thousand years. It is increasingly being used not just as a traditional herbal medicine but also as a popular functional food. In this study, qualitative and quantitative analysis of PR from three different origins were initially performed using chemical fingerprint and chemometrics methods. Hierarchical cluster analysis (HCA) and Principal component analysis (PCA) were used to classify 60 PR samples from three different origins. The results revealed that the PR samples fell into three clusters related to the origins. In addition, pairwise comparison of varying PR and obtaining chemical markers between different species through the establishment of partial least squares discriminant analysis. Finally, chemical markers 9,13 and 17 were identified by LC/MS as disporopsin, 5,7-dihydroxy-3-(4'-hydroxybenzyl)-6,8-dimethylchroman-4-one and (3R)-5,7-dihydroxy-3-(4'-hydroxybenzyl)-6-methylchroman-4-one or isomer, respectively. In conclusion, these methods can be applied to identify and distinguish the quality of PR with other original plants and provide novel ideas for evaluating herbal products used in TCM.
黄精作为一种传统中药已有两千年的历史。它不仅越来越多地被用作传统草药，而且还被用作一种受欢迎的功能性食品。在这项研究中，初步采用化学指纹图谱和化学计量学方法对来自三个不同产地的黄精进行定性和定量分析。采用层次聚类分析（HCA）和主成分分析（PCA）对来自三个不同产地的 60 个黄精样品进行分类。结果表明，黄精样品分为与产地相关的三个聚类。此外，通过建立偏最小二乘判别分析，对不同产地的黄精进行两两比较，并获得不同种之间的化学标志物。最后，通过 LC/MS 将化学标志物 9、13 和 17 鉴定为二氢玉米黄质、5,7-二羟基-3-(4'-羟基苄基)-6,8-二甲基色烷-4-酮和（3R）-5,7-二羟基-3-(4'-羟基苄基)-6-甲基色烷-4-酮或其异构体。总之，这些方法可用于鉴定和区分其他原植物来源的黄精质量，并为中药中使用的草药产品评价提供新的思路。
