College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
School of Medicine, Changchun Sci-Tech University, Changchun 130600, China.
Molecules. 2024 Sep 27;29(19):4600. doi: 10.3390/molecules29194600.
As a traditional Chinese medicine (TCM), Allii Macrostemonis Bulbus (AMB) is a key herb for the treatment of thoracic paralytic cardiac pain, but its quality evaluation method has not yet been fully clarified. In this study, chromatographic fingerprints of AMB were developed using solid-phase extraction-high-performance liquid chromatography-evaporative light scattering detection (SPE-HPLC-ELSD) to evaluate the quality of AMB from various origins and processing methods. This was achieved by employing chemical pattern recognition techniques and verifying the feasibility and applicability of the quality evaluation of AMB through the quantitative analysis of multi-components via a single-marker (QAMS) method. Through the analysis of the fingerprints of 18 batches of AMB, 30 common peaks were screened, and 6 components (adenosine, syringin, macrostemonoside T, macrostemonoside A, macrostemonoside U, and macrostemonoside V) were identified. Moreover, three differential markers (macrostemonoside A, macrostemonoside T, and macrostemonoside U) were screened out using chemometrics techniques, including principal component analysis (PCA), hierarchical cluster analysis (HCA), and orthogonal partial least squares discriminant analysis (OPLS-DA). Subsequently, a QAMS method was established for macrostemonoside T and macrostemonoside U using macrostemonoside A as an internal reference. The results demonstrate the method's accuracy, reproducibility, and stability, rendering it suitable for the quality evaluation of AMB. This study provides a theoretical basis for drug quality control and the discovery of quality markers for AMB.
作为一种传统中药（TCM），Allii Macrostemonis Bulbus（AMB）是治疗胸痹心痛的关键草药，但尚未充分阐明其质量评价方法。本研究采用固相萃取-高效液相色谱-蒸发光散射检测（SPE-HPLC-ELSD）法建立 AMB 的色谱指纹图谱，以评估不同来源和加工方法的 AMB 的质量。通过化学模式识别技术，并通过通过单一标记物（QAMS）法对多成分进行定量分析来验证 AMB 质量评价的可行性和适用性，实现了这一目标。通过对 18 批 AMB 的指纹图谱分析，筛选出 30 个共有峰，并鉴定出 6 个成分（腺苷、丁香苷、毛蕊异黄酮苷 T、毛蕊异黄酮苷 A、毛蕊异黄酮苷 U 和毛蕊异黄酮苷 V）。此外，采用主成分分析（PCA）、层次聚类分析（HCA）和正交偏最小二乘判别分析（OPLS-DA）等化学计量学技术筛选出 3 个差异标志物（毛蕊异黄酮苷 A、毛蕊异黄酮苷 T 和毛蕊异黄酮苷 U）。随后，建立了以毛蕊异黄酮苷 A 为内标物的毛蕊异黄酮苷 T 和毛蕊异黄酮苷 U 的 QAMS 方法。结果表明该方法准确、重现性好、稳定性高，适用于 AMB 的质量评价。本研究为 AMB 的药物质量控制和质量标志物的发现提供了理论依据。
