Churkina L N, Stepaniuk V V, Kiprianova E A, Garagulia A D, Smirnov V V
Antibiot Med Biotekhnol. 1986 May;31(5):362-5.
The effect of antibiotic Al-87 on the ultrastructure of staphylococcal cells was studied. The cells of a control culture of a sensitive strain of S. aureus, 209P at the early exponential growth phase were characterized by thin walls (20-22 nm) and septa (30 nm). In the presence of the subbacteriostatic concentration of antibiotic AL-87 (0.02 microgram/ml) the thickness of the cell walls and septa increased up to 80-90 and 150 nm, respectively. Segregation of the septa was retarded and 4 cell conglomerates formed. The cell division appeared to be highly active: the septa were detected in 80-90 per cent of the sections against 40 per cent in the control. Therefore, antibiotic AL-87 induced significant thickening of the cell walls and impairment of the cell division regulation. Investigation of the staphylococcal variant resistant to the antibiotic showed that there were no significant differences between the cells grown in the absence and presence of antibiotic AL-87 (in a concentration of 200 micrograms/ml). In both the experiments there were detected cells in their majority with thinner walls, L-form-like structures, protoplasts and single conglomerates of the cells with thicker walls and anomalous division and the cells at the moment of lysis. It suggested that the effect of antibiotic AL-87 on the cell wall structure was not direct i. e. by inhibition of protein synthesis but mediated i. e. due to shifts in lipid synthesis inducing changes in lipid-dependent synthesis of the cell wall polymers.
研究了抗生素Al - 87对葡萄球菌细胞超微结构的影响。金黄色葡萄球菌敏感菌株209P处于指数生长早期的对照培养物细胞，其细胞壁薄（20 - 22纳米），隔膜厚（30纳米）。在亚抑菌浓度的抗生素AL - 87（0.02微克/毫升）存在下，细胞壁和隔膜的厚度分别增加到80 - 90纳米和150纳米。隔膜的分离受阻，形成了4个细胞聚集体。细胞分裂似乎非常活跃：在80 - 90%的切片中检测到隔膜，而对照中为40%。因此，抗生素AL - 87导致细胞壁显著增厚并损害细胞分裂调节。对该抗生素耐药的葡萄球菌变体的研究表明，在不存在和存在抗生素AL - 87（浓度为200微克/毫升）的情况下生长的细胞之间没有显著差异。在两个实验中都检测到大多数细胞具有较薄的壁、L型样结构、原生质体以及具有较厚壁和异常分裂的单个细胞聚集体，还有正在裂解的细胞。这表明抗生素AL - 87对细胞壁结构的影响不是直接的，即不是通过抑制蛋白质合成，而是间接介导的，即由于脂质合成的改变导致依赖脂质的细胞壁聚合物合成发生变化。
