Sharma Neha, Dabral Surbhi, Tyagi Jaagriti, Yadav Gaurav, Aggarwal Himanshi, Joshi Naveen Chandra, Varma Ajit, Koul Monika, Choudhary Devendra Kumar, Mishra Arti
Amity Institute of Microbial Technology, Amity University, Uttar Pradesh, India.
Department of Botany, Hansraj College, University of Delhi, Delhi, India.
Front Plant Sci. 2023 May 24;14:1155715. doi: 10.3389/fpls.2023.1155715. eCollection 2023.
Rapid urbanization and globalization demand increasing agricultural productivity. Soil nutrient supply capacity is continuously decreasing due to soil erosion, degradation, salt deposition, undesired element, metal deposition, water scarcity, and an uneven nutrient delivery system. Rice cultivation requires a large amount of water which is becoming detrimental due to these activities. There is a need to increase its productivity. Microbial inoculants are becoming increasingly important in achieving sustainable agricultural production systems. The current study was conducted to investigate the interaction between the root endophytic fungus () and the actinobacterium sp. ISTPL4 (Z. sp. ISTPL4) and their synergistic effects on the growth of rice (Oryza sativa L). Both and Z. sp. ISTPL4 showed positive interactions. Growth of S. indica was observed at different days after Z. sp. ISTPL4 inoculation, and stimulated growth of was observed when Z. sp. ISTPL4 was inoculated at 5 dafi (days after fungal inoculation). Z. sp. ISTPL4 promoted the growth of S. indica as it increased spore germination. Furthermore, confocal and scanning electron microscopy (SEM) analyses showed a 27% increase in the spore size of S. indica in the presence of Z. sp. ISTPL4. In a liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis increased production of alanine and glutamic acid was observed in their sequential co-culture as compared with individual cultures. Sequential inoculation of and Z. sp. ISTPL4 significantly increased the biochemical and physical characteristics of rice as compared with their individual inoculum. Biochemical parameters such as chlorophyll content, total soluble sugar, and flavonoid content in the rice increased by up to 57%, 47%, and 39%, respectively, in the presence of the combined inoculum of and Z. sp. ISTPL4. This will be the first study, to the best of our knowledge, which shows the fungus and actinobacterium interaction and their synergistic roles in the growth promotion of rice. Furthermore, this novel combination can also be used to boost the growth of other crops to increase the agricultural yield.
快速的城市化和全球化要求提高农业生产力。由于土壤侵蚀、退化、盐分沉积、有害元素、金属沉积、水资源短缺以及养分输送系统不均衡，土壤养分供应能力持续下降。水稻种植需要大量的水，而由于上述这些活动，这正变得有害。有必要提高其生产力。微生物接种剂在实现可持续农业生产系统方面正变得越来越重要。当前的研究旨在调查根内生真菌（）与放线菌ISTPL4菌株（Z. sp. ISTPL4）之间的相互作用及其对水稻（Oryza sativa L）生长的协同效应。和Z. sp. ISTPL4均表现出积极的相互作用。在接种Z. sp. ISTPL4后的不同天数观察到了稻瘟病菌的生长，并且当在真菌接种后第5天接种Z. sp. ISTPL4时，观察到稻瘟病菌的生长受到刺激。Z. sp. ISTPL4促进了稻瘟病菌的生长，因为它增加了孢子萌发。此外，共聚焦和扫描电子显微镜（SEM）分析表明，在存在Z. sp. ISTPL4的情况下，稻瘟病菌的孢子大小增加了27%。在液相色谱 - 串联质谱（LC - MS/MS）分析中，与单独培养相比，在它们的顺序共培养中观察到丙氨酸和谷氨酸的产量增加。与单独接种相比，顺序接种和Z. sp. ISTPL4显著提高了水稻的生化和物理特性。在和Z. sp. ISTPL4的联合接种物存在的情况下，水稻中的生化参数如叶绿素含量、总可溶性糖和类黄酮含量分别增加了高达57%、47%和39%。据我们所知，这将是第一项展示真菌与放线菌相互作用及其在促进水稻生长中的协同作用的研究。此外，这种新型组合还可用于促进其他作物的生长以提高农业产量。
