State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China.
Haihe Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China.
J Agric Food Chem. 2023 Jun 21;71(24):9391-9403. doi: 10.1021/acs.jafc.3c01093. Epub 2023 Jun 9.
Ginseng extracts are extensively used as raw materials for food supplements and herbal medicines. This study aimed to characterize ginsenosides obtained from six plant extracts (, red ginseng, , , , and var. ) and compared them with their metabolic profiles mediated by rat intestinal microbiota. Ultrahigh-performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (UHPLC/IM-QTOF-MS) with scheduled multiple reaction monitoring (sMRM) quantitation methods were developed to characterize and compare the ginsenoside composition of the different extracts. After incubation, 248 ginsenosides/metabolites were identified by UHPLC/IM-QTOF-MS in six biotransformed samples. Deglycosylation was determined to be the main metabolic pathway of ginsenosides, and protopanaxadiol-type and oleanolic acid-type saponins were easier to be easily metabolized. Compared with the ginsenosides in plant extracts, those remaining in six biotransformed samples were considerably fewer after biotransformation for 8 h. However, the compositional differences in four subtypes of the ginsenosides among the six plants became more distinct.
人参提取物被广泛用作食品补充剂和草药的原料。本研究旨在对六种植物提取物（生晒参、红参、白参、西洋参、三七和三七花）中的人参皂苷进行表征，并比较它们在大鼠肠道微生物群介导下的代谢谱。建立了超高效液相色谱/离子淌度-四极杆飞行时间质谱（UHPLC/IM-QTOF-MS）与预定多重反应监测（sMRM）定量方法，以对不同提取物中的人参皂苷组成进行表征和比较。孵育后，通过 UHPLC/IM-QTOF-MS 在六种生物转化样品中鉴定出 248 个人参皂苷/代谢物。研究发现，去糖基化是人参皂苷的主要代谢途径，原人参二醇型和齐墩果酸型皂苷更容易被代谢。与植物提取物中的人参皂苷相比，经过 8 小时生物转化后，六种生物转化样品中剩余的人参皂苷数量明显减少。然而，在四种亚型的人参皂苷中，六种植物之间的成分差异变得更加明显。
