Department of Materials Engineering, Indian Institute of Science (IISc), Bengaluru, Karnataka 560012, India.
College of Science, Engineering and Technology, University of South Africa, Florida, Johannesburg 1709, South Africa.
ACS Biomater Sci Eng. 2023 Jul 10;9(7):4208-4222. doi: 10.1021/acsbiomaterials.3c00392. Epub 2023 Jun 9.
This study reports the novel use of () mucus as a potential therapeutic repair agent in osteoarthritis and cartilage tissue repair . Snail mucus was isolated, sterilized, and characterized using FTIR, XPS, rheology, and LC-MS/MS. The GAGs, sugar, phenol, and protein contents were estimated using standard assays. The LC-MS/MS identified 6-gingerol and some other small molecules. The effects of the sterilized mucus were studied on human chondrocytes using the C28/I2 cell as a model for the assays. The MTT assay indicates that mucus extracted from the pedal of is biocompatible with the cells up to a concentration of 50 μg/mL. The mucus promoted cell migration and proliferation and completely closed the wound within 72 h, as indicated in the scratch assay. In addition, the snail mucus reduced apoptosis significantly ( < 0.05) in the treated cells by 74.6%. It preserved the cytoskeletal integrity of the C28/I2 cells, attributed mainly to GAGs and 6-gingerol content of the mucus. In conclusion, this present study suggests that GAGs and 6-gingerol conferred wound-healing and antiapoptotic properties on the mucus secretion from and can be explored for therapeutic repair and cartilage tissue engineering.
本研究报告了一种新型的（）黏液，作为骨关节炎和软骨组织修复的潜在治疗修复剂。蜗牛黏液被分离、消毒，并通过傅里叶变换红外光谱（FTIR）、X 射线光电子能谱（XPS）、流变学和液质联用（LC-MS/MS）进行了表征。使用标准测定法估计糖胺聚糖（GAGs）、糖、酚和蛋白质含量。LC-MS/MS 鉴定出 6-姜酚和一些其他小分子。使用 C28/I2 细胞作为模型，对经消毒的黏液对人软骨细胞的影响进行了研究。MTT 测定表明，从蜗牛足部提取的黏液与细胞在 50μg/mL 的浓度下具有生物相容性。在划痕实验中，黏液促进细胞迁移和增殖，并在 72 小时内完全封闭伤口。此外，蜗牛黏液通过减少细胞凋亡（<0.05）达到 74.6%。它保持了 C28/I2 细胞的细胞骨架完整性，这主要归因于黏液中的 GAGs 和 6-姜酚含量。总之，本研究表明，GAGs 和 6-姜酚赋予了蜗牛黏液的伤口愈合和抗凋亡特性，可用于治疗修复和软骨组织工程。
