Inaba M, Nagashima K
Gan To Kagaku Ryoho. 1986 Jul;13(7):2337-41.
In order to investigate the possible mechanism for the therapeutic efficacy of tetracycline antibiotics against malignant pleural effusion, the effect of minocycline (MC), one of this type of antibiotic, on in vitro growth of tumor cells was examined. As a result, it was found that MC caused complete growth inhibition of various tumor cell lines at concentration of 10-20 micrograms/ml, but this action was reversible, suggesting that considerably long exposure time would be needed for these drugs to kill the tumor cells in vivo at a relatively low concentration. On the other hand, when a relatively high concentration of 100 micrograms/ml was applied, MC induced irreversible inhibition of cell growth even if the exposure time was comparatively short. Since 4 human lung tumor cell lines examined exhibited high sensitivity to these antibiotics, it seems possible that their direct administration in the form of a high-concentration solution into the pleural cavity would result in a direct cytostatic effect on tumor cells in clinical therapy.
为了探究四环素类抗生素治疗恶性胸腔积液的可能机制，研究了该类抗生素之一米诺环素（MC）对肿瘤细胞体外生长的影响。结果发现，MC在浓度为10 - 20微克/毫升时可导致各种肿瘤细胞系完全生长抑制，但这种作用是可逆的，这表明在体内以相对低浓度使用这些药物杀死肿瘤细胞需要相当长的暴露时间。另一方面，当应用100微克/毫升的相对高浓度时，即使暴露时间相对较短，MC也会诱导细胞生长的不可逆抑制。由于所检测的4种人肺肿瘤细胞系对这些抗生素表现出高敏感性，因此在临床治疗中以高浓度溶液形式直接注入胸腔似乎可能对肿瘤细胞产生直接的细胞抑制作用。
