Wan J H, Zhang S J, Mi J X, Qi S L, Wu K F, Gan F, Yang B Q, Xu L J, Xu L Z
Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin.
Sci China B. 1989 Sep;32(9):1087-98.
An in vitro-vivo technique for establishment of cell lines on murine leukemia has been developed. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, B lymphocytic leukemia P 388-86 and Friend erythroleukemia FLCL cell lines have been established. Incidence of leukemia with these cell lines was 100%. Along with the increase of generations of cell lines, cell growth accelerated, generation time shortened and cloning efficiencies rose. A following up electron microscopic observation on L7811-85 and L7212-85 showed that the virus particles were "A" particles in original cells. When they became cell lines in vitro, virus particles increased and transformed into typical "C" particles with budding. An inhibitory activity relevant to leukemic cells on proliferation of leukemic cells has been observed in the supernatant of L7811-85 medium and was regarded as an "autocrine".
已开发出一种用于在小鼠白血病上建立细胞系的体外-体内技术。使用该方法，已建立了抑制性T淋巴细胞白血病L7811-85、L7212-85，非T、非B淋巴细胞白血病L1210-86，B淋巴细胞白血病P 388-86和Friend红白血病FLCL细胞系。这些细胞系诱发白血病的发生率为100%。随着细胞系传代次数的增加，细胞生长加速，代时缩短，克隆效率提高。对L7811-85和L7212-85进行的后续电子显微镜观察表明，原始细胞中的病毒颗粒为“A”颗粒。当它们在体外成为细胞系时，病毒颗粒增加并转化为典型的有芽生的“C”颗粒。在L7811-85培养基的上清液中观察到与白血病细胞相关的对白血病细胞增殖的抑制活性，并将其视为一种“自分泌”现象。
