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铝盐和铁盐对 BSA 溶液干燥成膜后之之字形图案参数的影响。

Influence of aluminum and iron chlorides on the parameters of zigzag patterns on films dried from BSA solutions.

机构信息

O. Ya. Usikov Institute for Radiophysics and Electronics, National Academy of Sciences of Ukraine, 12 Academician Proskura Str., Kharkiv, 61085, Ukraine.

V. N. Karazin Kharkiv National University, 4 Svobody Sq., Kharkiv, 61022, Ukraine.

出版信息

Sci Rep. 2023 Jun 9;13(1):9426. doi: 10.1038/s41598-023-36515-4.

Abstract

The relationships between the structural and aggregational state of bovine serum albumin (BSA) and the specific length and total number of zigzag pattern segments of the film textures formed upon drying biopolymer solutions with aluminum and iron chlorides have been shown. To obtain films, saline solutions of BSA were dried in a glass cuvette under thermostatically controlled conditions. It is shown that the formation of zigzag structures is sensitive to the influence of aluminum chlorides Al and iron chlorides Fe and depend on the concentration of AlCl and FeCl. This may be due to a change in the charge and size of BSA particles and due to a change in conformation or a violation of the structure of BSA. These factors, in turn, affect the hydration of the solution components and the structural state of free water in solution, which presumably also affects the formation of zigzag structures. It is established that the analysis of the specific length and the number of segments of zigzag patterns makes it possible to evaluate changes in the state of biopolymers in the initial solution during structural changes and aggregation.

摘要

已经证明了牛血清白蛋白(BSA)的结构和聚集状态与在干燥含有铝和铁氯化物的生物聚合物溶液时形成的薄膜纹理的特定长度和锯齿形图案段总数之间的关系。为了获得薄膜,将 BSA 的盐溶液在玻璃比色皿中在恒温控制条件下干燥。结果表明,锯齿形结构的形成对铝氯化物 Al 和铁氯化物 Fe 的影响很敏感,并且取决于 AlCl 和 FeCl 的浓度。这可能是由于 BSA 颗粒的电荷和大小发生变化,以及构象发生变化或 BSA 结构遭到破坏。这些因素反过来又影响溶液成分的水合作用和溶液中游离水的结构状态,这可能也会影响锯齿形结构的形成。已经确定,分析锯齿形图案的特定长度和段数,可以在结构变化和聚集过程中评估初始溶液中生物聚合物的状态变化。

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