Department of Bioresource Sciences, Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
Biosci Biotechnol Biochem. 2023 Aug 23;87(9):1045-1055. doi: 10.1093/bbb/zbad078.
Binding of the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to the cognate angiotensin-converting enzyme 2 (ACE2) receptor is the initial step in the viral infection process. In this study, we screened an in-house extract library to identify food materials with inhibitory activity against this binding using enzyme-linked immunosorbent assays and attempted to ascertain their active constituents. Hydrangea macrophylla var. thunbergia leaves were identified as candidate materials. Its active compounds were purified using conventional chromatographic methods and identified as naringenin, dihydroisocoumarins, hydrangenol, and phyllodulcin, which have affinities for the ACE2 receptor and inhibit ACE2 receptor-spike S1 binding. Given that boiled water extracts of H. macrophylla leaves are commonly consumed as sweet tea in Japan, we speculated that this tea could be used as a potential natural resource to reduce the risk of SARS-CoV-2 infection.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的刺突蛋白与同源血管紧张素转换酶 2（ACE2）受体的结合是病毒感染过程中的初始步骤。在这项研究中，我们使用酶联免疫吸附测定法筛选了内部提取物文库，以鉴定具有抑制这种结合活性的食物材料，并试图确定其有效成分。绣球大八仙花变种的叶片被鉴定为候选材料。其活性化合物使用常规色谱方法进行纯化，并鉴定为柚皮素、二氢异香豆素、羟基根皮苷和根皮苷，它们对 ACE2 受体具有亲和力，并抑制 ACE2 受体-刺突 S1 结合。鉴于绣球大八仙花叶片的沸水提取物在日本通常被用作甜茶，我们推测这种茶可能被用作降低 SARS-CoV-2 感染风险的潜在天然资源。
