The Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, 443002, China.
The Third-Grade Pharmacological Laboratory On Traditional, Chinese Medicine (Approved By State Administration of Traditional Chinese Medicine), China Three Gorges University, Yichang, 443002, China.
Curr Microbiol. 2023 Jun 9;80(8):240. doi: 10.1007/s00284-023-03352-7.
Improving the utilization rate of loaded-drugs is of huge importance for generating chitosan-based (CS) micro-carriers. This study aims to fabricate a novel CS microspheres co-delivered curcumin (Cur) and gallic acid (Ga) to assess drug loading and release kinetics, the blood compatibility and anti-osteosarcoma properties. The present study observes the interaction between CS and Cur/Ga molecules and estimates the change in crystallinity and loading and release rate. In addition, blood compatibility and cytotoxicity of such microspheres are also evaluated. Cur-Ga-CS microspheres present high entrapment rate of (55.84 ± 0.34) % for Ga and (42.68 ± 0.11) % for Cur, possibly attributed to surface positive charge (21.76 ± 2.46) mV. Strikingly, Cur-Ga-CS microspheres exhibit slowly sustainable release for almost 7 days in physiological buffer. Importantly, these microspheres possess negligibly toxic to blood and normal BMSC cells, but strong anti-osteosarcoma effect on U2OS cells. Overall, Cur-Ga-CS microspheres are promising to become a novel anti-osteosarcoma agent or sustainable delivery carrier in biomedical applications.
提高载药微球的利用率对于壳聚糖（CS）基微载体的生成至关重要。本研究旨在制备一种新型的姜黄素（Cur）和没食子酸（Ga）共载的 CS 微球，以评估其载药和释放动力学、血液相容性和抗骨肉瘤特性。本研究观察了 CS 与 Cur/Ga 分子之间的相互作用，并估算了结晶度变化、载药量和释放率的变化。此外，还评估了此类微球的血液相容性和细胞毒性。Cur-Ga-CS 微球对 Ga 的包封率（55.84 ± 0.34）%，对 Cur 的包封率（42.68 ± 0.11）%较高，这可能归因于表面带正电荷（21.76 ± 2.46）mV。值得注意的是，Cur-Ga-CS 微球在生理缓冲液中几乎可持续释放 7 天。重要的是，这些微球对血液和正常 BMSC 细胞几乎没有毒性，但对 U2OS 细胞具有很强的抗骨肉瘤作用。总的来说，Cur-Ga-CS 微球有望成为一种新型的抗骨肉瘤药物或生物医学应用中的可持续输送载体。
