Suppr超能文献

意大利南部儿科家庭医生队列中对 SARS-CoV-2 疫苗的免疫反应。

The Immune Response to SARS-CoV-2 Vaccine in a Cohort of Family Pediatricians from Southern Italy.

机构信息

Federazione Italiana Medici Pediatri (FIMP), 80142 Naples, Italy.

Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, 80131 Naples, Italy.

出版信息

Cells. 2023 May 23;12(11):1447. doi: 10.3390/cells12111447.

Abstract

In Italy, from January 2021, the Ministry of Health indicated a vaccination plan against COVID for frail patients and physicians based on a three-dose scheme. However, conflicting results have been reported on which biomarkers permit immunization assessment. We used several laboratory approaches (i.e., antibodies serum levels, flow cytometry analysis, and cytokines release by stimulated cells) to investigate the immune response in a cohort of 53 family pediatricians (FPs) at different times after the vaccine. We observed that the BNT162b2-mRNA vaccine induced a significant increase of specific antibodies after the third (booster) dose; however, the antibody titer was not predictive of the risk of developing the infection in the six months following the booster dose. The antigen stimulation of PBMC cells from subjects vaccinated with the third booster jab induced the increase of the activated T cells (i.e., CD4 CD154); the frequency of CD4 CD154 TNF-α cells, as well as the TNF-α secretion, was not modified, while we observed a trend of increase of IFN-γ secretion. Interestingly, the level of CD8 IFN-γ (independently from antibody titer) was significantly increased after the third dose and predicts the risk of developing the infection in the six months following the booster jab. Such results may impact also other virus vaccinations.

摘要

在意大利,自 2021 年 1 月以来,卫生部根据三剂方案为体弱患者和医生制定了针对 COVID 的疫苗接种计划。然而,关于哪些生物标志物可以进行免疫评估,报告的结果存在冲突。我们使用了几种实验室方法(即抗体血清水平、流式细胞术分析和刺激细胞释放的细胞因子)来研究 53 名家庭儿科医生(FPs)在接种疫苗后的不同时间的免疫反应。我们观察到,BNT162b2-mRNA 疫苗在第三剂(加强针)后诱导了特异性抗体的显著增加;然而,抗体滴度不能预测在加强针接种后六个月内感染的风险。用第三剂加强针接种的受试者的 PBMC 细胞的抗原刺激诱导了激活的 T 细胞(即 CD4 CD154)的增加;CD4 CD154 TNF-α 细胞的频率以及 TNF-α 的分泌没有改变,而我们观察到 IFN-γ 分泌增加的趋势。有趣的是,第三剂后 CD8 IFN-γ 的水平(独立于抗体滴度)显著增加,并预测在加强针接种后六个月内感染的风险。这些结果可能对其他病毒疫苗接种也有影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2862/10252549/09641a3c8fd7/cells-12-01447-g001.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验