Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland.
Int J Mol Sci. 2023 May 30;24(11):9468. doi: 10.3390/ijms24119468.
The rapid increase in disease prevalence in the world makes it extremely important to search for new or develop existing diagnostic methods, for example, chemiluminescent labeling used in immunodiagnostics. At present, acridinium esters are willingly used as chemiluminogenic fragments of labels. However, the search for new chemiluminogens that are particularly efficient is the main task of our studies. The density functional theory (DFT) and time-dependent (TD) DFT methods were used to obtain thermodynamic and kinetic results concerning the chemiluminescence and competitive dark reactions, which indicated whether some of the scrutinized derivatives have better characteristics than the chemiluminogens used so far. Synthesis of these candidates for efficient chemiluminogens, followed by studies of their chemiluminescent properties, and ultimately in chemiluminescent labeling, are further steps to confirm their potential applicability in immunodiagnostics.
世界上疾病流行率的迅速增加使得寻找新的或开发现有的诊断方法变得极其重要，例如免疫诊断中使用的化学发光标记。目前，吖啶酯类化合物被欣然用作标记的化学发光基团。然而，寻找特别有效的新化学发光物是我们研究的主要任务。密度泛函理论（DFT）和含时（TD）DFT 方法用于获得有关化学发光和竞争暗反应的热力学和动力学结果，这些结果表明一些被仔细研究的衍生物是否具有比迄今为止使用的化学发光物更好的特性。这些高效化学发光物候选物的合成，随后对其化学发光性质的研究，最终在化学发光标记中的应用，是进一步确认它们在免疫诊断中潜在适用性的步骤。
