鲁米诺/核黄素化学发光及其在选择性和灵敏多巴胺检测中的应用。
Chemiluminescence of Lucigenin/Riboflavin and Its Application for Selective and Sensitive Dopamine Detection.
机构信息
College of Chemistry and Bioengineering , Guilin University of Technology , Guilin 541004 , China.
State Key Laboratory of Electroanalytical Chemistry , Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun , Jilin 130022 , PR China.
出版信息
Anal Chem. 2019 Feb 5;91(3):2135-2139. doi: 10.1021/acs.analchem.8b04670. Epub 2019 Jan 10.
Lucigenin-riboflavin chemiluminescence is reported for the first time. Moreover, most dopamine chemiluminescence (CL) detection methods are based on the quenching of CL by dopamine. In contrast, we find that dopamine can significantly enhance lucigenin/riboflavin CL and establish a highly sensitive turn-on method for dopamine detection based on the enhancement of lucigenin/riboflavin CL. Under the optimal conditions, the CL intensity of the lucigenin/riboflavin system increased linearly with the concentration of dopamine in the range of 0.0056-55.56 μM, and the limit of detection is 1.87 nM.
首次报道了鲁米诺-核黄素化学发光。此外，大多数多巴胺化学发光（CL）检测方法都是基于多巴胺对 CL 的猝灭。相比之下，我们发现多巴胺可以显著增强鲁米诺/核黄素 CL，并基于增强的鲁米诺/核黄素 CL 建立了一种用于多巴胺检测的高灵敏度开启方法。在最佳条件下，鲁米诺/核黄素体系的 CL 强度随多巴胺浓度在 0.0056-55.56 μM 范围内呈线性增加，检测限为 1.87 nM。
Zotero 插件