Aquaculture Department, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Research Center of Aquatic Animal Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2023 May 30;24(11):9518. doi: 10.3390/ijms24119518.
Nucleotide-binding and oligomerization domain-like receptors (NOD-like receptors, NLRs) can regulate the inflammatory response to eliminate pathogens and maintain the host's homeostasis. In this study, the head kidney macrophages of Siberian sturgeon were treated with lipopolysaccharide (LPS) to induce inflammation by evaluating the expression of cytokines. The high-throughput sequencing for macrophages after 12 h treatment showed that 1224 differentially expressed genes (DEGs), including 779 upregulated and 445 downregulated, were identified. DEGs mainly focus on pattern recognition receptors (PRRs) and the adaptor proteins, cytokines, and cell adhesion molecules. In the NOD-like receptor signaling pathway, multiple NOD-like receptor family CARD domains containing 3-like (NLRC3-like) were significantly downregulated, and pro-inflammatory cytokines were upregulated. Based on the transcriptome database, 19 NLRs with NACHT structural domains were mined and named in Siberian sturgeon, including 5 NLR-A, 12 NLR-C, and 2 other NLRs. The NLR-C subfamily had the characteristics of expansion of the teleost NLRC3 family and lacked the B30.2 domain compared with other fish. This study revealed the inflammatory response mechanism and NLRs family characterization in Siberian sturgeon by transcriptome and provided basic data for further research on inflammation in teleost.
核苷酸结合寡聚化结构域样受体（NOD-like receptors，NLRs）可以调节炎症反应，以消除病原体并维持宿主的内稳态。本研究通过评估细胞因子的表达，用脂多糖（LPS）处理西伯利亚鱘头肾巨噬细胞以诱导炎症。处理 12 h 后巨噬细胞的高通量测序显示，鉴定出 1224 个差异表达基因（DEGs），包括 779 个上调和 445 个下调。DEGs 主要集中在模式识别受体（PRRs）和衔接蛋白、细胞因子和细胞黏附分子上。在 NOD 样受体信号通路中，多个 NOD 样受体家族 CARD 结构域包含 3 样（NLRC3-like）显著下调，促炎细胞因子上调。基于转录组数据库，在西伯利亚鱘中挖掘并命名了 19 个具有 NACHT 结构域的 NLRs，包括 5 个 NLR-A、12 个 NLR-C 和 2 个其他 NLRs。NLR-C 亚家族具有扩展的硬骨鱼 NLRC3 家族的特征，与其他鱼类相比缺乏 B30.2 结构域。本研究通过转录组揭示了西伯利亚鱘的炎症反应机制和 NLRs 家族特征，为进一步研究硬骨鱼的炎症提供了基础数据。
