Department of Biochemistry, Medical Academy, Lithuanian University of Health Sciences, A. Mickeviciaus St. 9, LT-44307 Kaunas, Lithuania.
Neuroscience Institute, Lithuanian University of Health Sciences, Eiveniu St. 4, LT-50009 Kaunas, Lithuania.
Int J Mol Sci. 2023 Jun 2;24(11):9704. doi: 10.3390/ijms24119704.
(1) In this study we determined the effect of long-term selenomethionine administration on the oxidative stress level and changes in antioxidant protein/enzyme activity; mRNA expression; and the levels of iron, zinc, and copper. (2) Experiments were performed on 4-6-week-old BALB/c mice, which were given selenomethionine (0.4 mg Se/kg b.w.) solution for 8 weeks. The element concentration was determined via inductively coupled plasma mass spectrometry. mRNA expression of , , and was quantified using real-time quantitative reverse transcription. Malondialdehyde content and catalase activity were determined spectrophotometrically. (3) After long-term SeMet administration, the amount of Se increased by 12-fold in mouse blood, 15-fold in the liver, and 42-fold in the brain, as compared to that in the control. Exposure to SeMet decreased amounts of Fe and Cu in blood, but increased Fe and Zn levels in the liver and increased the levels of all examined elements in the brain. Se increased malondialdehyde content in the blood and brain but decreased it in liver. SeMet administration increased the mRNA expression of selenoprotein P, dismutase, and catalase, but decreased catalase activity in brain and liver. (4) Eight-week-long selenomethionine consumption elevated Se levels in the blood, liver, and especially in the brain and disturbed the homeostasis of Fe, Zn, and Cu. Moreover, Se induced lipid peroxidation in the blood and brain, but not in the liver. In response to SeMet exposure, significant up-regulation of the mRNA expression of catalase, superoxide dismutase 1, and selenoprotein P in the brain, and especially in the liver, was determined.
(1) 在这项研究中，我们确定了长期给予硒蛋氨酸对氧化应激水平和抗氧化蛋白/酶活性变化的影响；mRNA 表达；以及铁、锌和铜的水平。(2) 实验在 4-6 周龄 BALB/c 小鼠上进行，给予硒蛋氨酸（0.4mg Se/kg b.w.）溶液 8 周。通过电感耦合等离子体质谱法测定元素浓度。使用实时定量逆转录定量测定 、 、和 的 mRNA 表达。通过分光光度法测定丙二醛含量和过氧化氢酶活性。(3) 长期给予 SeMet 后，与对照组相比，小鼠血液中的硒含量增加了 12 倍，肝脏中的硒含量增加了 15 倍，大脑中的硒含量增加了 42 倍。暴露于 SeMet 降低了血液中的铁和铜含量，但增加了肝脏中的铁和锌水平，并增加了大脑中所有检测元素的水平。硒增加了血液和大脑中的丙二醛含量，但降低了肝脏中的丙二醛含量。SeMet 给药增加了脑和肝中硒蛋白 P、超氧化物歧化酶和过氧化氢酶的 mRNA 表达，但降低了脑和肝中过氧化氢酶的活性。(4) 8 周的硒蛋氨酸消耗提高了血液、肝脏中硒的水平，特别是在大脑中，并扰乱了铁、锌和铜的体内平衡。此外，硒在血液和大脑中诱导脂质过氧化，但在肝脏中没有。暴露于 SeMet 后，确定了脑，特别是肝中过氧化氢酶、超氧化物歧化酶 1 和硒蛋白 P 的 mRNA 表达显著上调。
