Medical College, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Key Laboratory of Human Functional Genomics of Jiangsu Province, Department of Neurobiology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 211166, China.
Molecules. 2023 May 31;28(11):4473. doi: 10.3390/molecules28114473.
Psoriasis is a chronic and multifactorial skin disease which is caused by inflammatory infiltrates, keratinocyte hyperproliferation, and accumulation of immune cells. As part of the species, Benzoylaconitine (BAC) shows potential antiviral, anti-tumor, and anti-inflammatory effects. In this study, we investigated the effects and mechanisms of BAC on tumor necrosis factor-alpha (TNF-α)/LPS-induced HaCaT keratinocytes in a imiquimod(IMQ)-induced mice model. The results showed that BAC could relieve the symptoms of psoriasis by inhibiting cell proliferation, the release of inflammatory factors, and the accumulation of Th17 cells, while no obvious effect on cell viability and safety was observed both in vitro and in vivo. Additionally, BAC can markedly inhibit the protein and mRNA levels of inflammatory cytokines in TNF-α/LPS-induced HaCaT keratinocytes by inhibiting the phosphorylation of STAT3. In brief, our data indicated that BAC could alleviate the progression of psoriasis and may be a potential therapeutic agent for treating psoriasis in clinical practice.
银屑病是一种慢性、多因素的皮肤疾病，其由炎症浸润、角质形成细胞过度增殖和免疫细胞积累引起。作为倍半萜生物碱的一种，苯甲酰乌头原碱（BAC）具有潜在的抗病毒、抗肿瘤和抗炎作用。在这项研究中，我们研究了 BAC 对咪喹莫特（IMQ）诱导的小鼠模型中肿瘤坏死因子-α（TNF-α）/脂多糖诱导的 HaCaT 角质形成细胞的作用及其机制。结果表明，BAC 可以通过抑制细胞增殖、炎症因子的释放和 Th17 细胞的积累来缓解银屑病的症状，同时在体外和体内均未观察到对细胞活力和安全性的明显影响。此外，BAC 可以通过抑制 STAT3 的磷酸化显著抑制 TNF-α/LPS 诱导的 HaCaT 角质形成细胞中炎症细胞因子的蛋白和 mRNA 水平。总之，我们的数据表明 BAC 可以缓解银屑病的进展，并且可能是临床治疗银屑病的一种潜在治疗药物。
