Lou Yuxia, Zhang Qiyu, Xu Qingyu, Yu Xinyu, Wang Wenxin, Gai Ruonan, Ming Feng
Development Centre of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Plants (Basel). 2023 Jun 2;12(11):2204. doi: 10.3390/plants12112204.
Flower breeders are continually refining their methods for producing high-quality flowers. species are considered the most important commercially grown orchids. Advances in genetic engineering technology have provided researchers with new tools that can be used along with traditional breeding methods to enhance floral traits and quality. However, the application of molecular techniques for the breeding of new species has been relatively rare. In this study, we constructed recombinant plasmids carrying flower color-related genes, Chalcone synthase () and/or Flavonoid 3',5'-hydroxylase (). These genes were transformed into both and plants using a gene gun or an -based method. Compared with WT, and both had deeper color and higher anthocyanin content in plants. Additionally, a phenotypic comparison with wild-type controls indicated the or -transgenic produced more branches, petals, and labial petals. Moreover, or -transgenic both showed deepened lip color, compared with the control. However, the intensity of the coloration of the lips decreased when protocorms were co-transformed with both and The results of this study confirm that and affect flower color in and may be relevant for the breeding of new orchid varieties with desirable flowering traits.
花卉育种者不断完善其培育高品质花卉的方法。蝴蝶兰物种被认为是商业种植最重要的兰花。基因工程技术的进步为研究人员提供了新工具，这些工具可与传统育种方法一起用于增强花卉性状和品质。然而，分子技术在新蝴蝶兰物种育种中的应用相对较少。在本研究中，我们构建了携带花色相关基因查尔酮合酶（CHS）和/或类黄酮3'，5'-羟化酶（F3'5'H）的重组质粒。这些基因通过基因枪或基于农杆菌的方法转化到蝴蝶兰和大花蕙兰植株中。与野生型相比，蝴蝶兰和大花蕙兰植株中的转基因植株都具有更深的颜色和更高的花青素含量。此外，与野生型对照的表型比较表明，转CHS或F3'5'H基因的蝴蝶兰产生了更多的分枝、花瓣和唇瓣。而且，与对照相比，转CHS或F3'5'H基因的大花蕙兰唇瓣颜色都加深了。然而，当原球茎同时用CHS和F3'5'H共转化时，大花蕙兰唇瓣的着色强度降低。本研究结果证实，CHS和F3'5'H影响蝴蝶兰和大花蕙兰的花色，可能与培育具有理想开花性状的新兰花品种有关。
