Hrazdina G, Weeden N F
Biochem Genet. 1986 Apr;24(3-4):309-17. doi: 10.1007/BF00502797.
Using enzymological and immunological methods we have investigated the relationship between chalcone synthase and the A locus, a major gene involved in the control of anthocyanin expression in pea (Pisum sativum L.) flowers. Pea plants containing the dominant allele A usually synthesize anthocyanins in the petal tissue, whereas plants homozygous for the a allele do not produce anthocyanins. We sought to determine whether or not the A locus also controlled the presence or absence of chalcone synthase, the first enzyme of the flavonoid pathway in the flowers of three genetic lines (A, purple-violet flowers; A,am, white flowers with sometimes pink edges; and a, white flowers). Chalcone synthase was found to be present in all three genetic lines by enzyme activity measurement, indirect enzyme-linked immunosorbent assay (ELISA), and Western blotting. Spectroscopic investigations showed that only the genetic lines A and A,am contained anthocyanins and flavonol glycosides, respectively, in the flowers; line a accumulated p-coumaric acid or its derivatives. These data suggest that the A locus in Pisum is not the structural gene for chalcone synthase and it does not appear to regulate the expression of this enzyme.
我们运用酶学和免疫学方法，研究了查尔酮合酶与A基因座之间的关系。A基因座是一个主要基因，参与调控豌豆（Pisum sativum L.）花中花青素的表达。含有显性等位基因A的豌豆植株通常在花瓣组织中合成花青素，而a等位基因纯合的植株则不产生花青素。我们试图确定A基因座是否也控制查尔酮合酶的有无，查尔酮合酶是三种遗传品系（A，紫罗兰花；A，am，有时带有粉色边缘的白花；以及a，白花）花朵中类黄酮途径的第一种酶。通过酶活性测定、间接酶联免疫吸附测定（ELISA）和蛋白质免疫印迹法，发现所有三种遗传品系中均存在查尔酮合酶。光谱研究表明，只有遗传品系A和A，am的花朵中分别含有花青素和黄酮醇糖苷；品系a积累了对香豆酸或其衍生物。这些数据表明，豌豆中的A基因座不是查尔酮合酶的结构基因，似乎也不调控该酶的表达。
