Sukum Pongphun, Punyodom Winita, Dangtip Somsak, Poramapijitwat Pipath, Daranarong Donraporn, Jenvoraphot Thannaphat, Nisoa Mudtorlep, Kuensaen Chakkrapong, Boonyawan Dheerawan
Doctor of Philosophy Program in Nanoscience and Nanotechnology (International Program/Interdisciplinary), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Center of Excellence in Materials Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand.
Polymers (Basel). 2023 Jun 2;15(11):2559. doi: 10.3390/polym15112559.
The wound-healing process can be disrupted at any stage due to various internal and external factors. The inflammatory stage of the process plays a vital role in determining the outcome of the wound. Prolonged inflammation due to bacterial infection can lead to tissue damage, slow healing, and complications. Wound dressings made using materials such as poly (vinyl alcohol) (PVA), chitosan (CS), and poly (ethylene glycol) (PEG) with Mangifera extract (ME) added can help reduce infection and inflammation, creating a conducive environment for faster healing. However, creating the electrospun membrane is challenging due to balancing various forces such as rheological behavior, conductivity, and surface tension. To improve the electrospinnability of the polymer solution, an atmospheric pressure plasma jet can induce chemistry in the solution and increase the polarity of the solvent. Thus, this research aims to investigate the effect of plasma treatment on PVA, CS, and PEG polymer solutions and fabricate ME wound dressing via electrospinning. The results indicated that increasing plasma treatment time increased the viscosity of the polymer solution, from 269 mPa∙to 331 mPa∙s after 60 min, and led to an increase in conductivity from 298 mS/cm to 330 mS/cm and an increase in nanofiber diameter from 90 ± 40 nm to 109 ± 49 nm. Incorporating 1% mangiferin extract into an electrospun nanofiber membrane has been found to increase the inhibition rates of and by 29.2% and 61.2%, respectively. Additionally, the fiber diameter decreases when compared with the electrospun nanofiber membrane without ME. Our findings demonstrate that electrospun nanofiber membrane with ME has anti-infective properties and can promote faster wound healing.
由于各种内部和外部因素，伤口愈合过程在任何阶段都可能受到干扰。该过程的炎症阶段在决定伤口的愈合结果方面起着至关重要的作用。细菌感染导致的炎症持续时间过长会导致组织损伤、愈合缓慢和并发症。使用聚（乙烯醇）（PVA）、壳聚糖（CS）和聚（乙二醇）（PEG）等材料并添加芒果提取物（ME）制成的伤口敷料有助于减少感染和炎症，为更快愈合创造有利环境。然而，由于要平衡流变行为、电导率和表面张力等各种力，制备电纺膜具有挑战性。为了提高聚合物溶液的可电纺性，大气压等离子体射流可以在溶液中引发化学反应并增加溶剂的极性。因此，本研究旨在研究等离子体处理对PVA、CS和PEG聚合物溶液的影响，并通过静电纺丝制备含ME的伤口敷料。结果表明，增加等离子体处理时间会增加聚合物溶液的粘度，60分钟后从269 mPa∙s增加到331 mPa∙s，并导致电导率从298 mS/cm增加到330 mS/cm，纳米纤维直径从90±40 nm增加到109±49 nm。已发现将1%的芒果苷提取物掺入电纺纳米纤维膜中可使金黄色葡萄球菌和大肠杆菌的抑制率分别提高29.2%和61.2%。此外，与不含ME的电纺纳米纤维膜相比，纤维直径减小。我们的研究结果表明，含ME的电纺纳米纤维膜具有抗感染特性，可促进伤口更快愈合。
