Department of Fisheries Science, Chonnam National University, Yeosu, South Korea.
Department of Fisheries Science, Chonnam National University, Yeosu, South Korea.
Mar Pollut Bull. 2023 Jul;192:115139. doi: 10.1016/j.marpolbul.2023.115139. Epub 2023 Jun 8.
Abalone are frequently exposed to several environmental factors including heavy metal toxicity, thermal stress, HO-stress, starvation, viral and bacterial infection that can induce oxidative stress. Glutathione reductase is a vital enzyme in the antioxidant defense system that catalyzes the reduction of oxidized glutathione to reduced glutathione. The present study aimed to identify and localize glutathione reductase in Pacific abalone (Hdh-GR) and assess its potential role in stress physiology, heavy metal toxicity, immune response, gonadal development, and metamorphosis. The mRNA expression of Hdh-GR was upregulated in response to thermal stress, starvation, HO-stress, and cadmium-exposed toxicity. The induced mRNA expression was also quantified in immune-challenged abalone. Moreover, the Hdh-GR expression was significantly higher during metamorphosis. The Hdh-GR mRNA expression showed an inverse relationship with ROS production in heat stressed Pacific abalone. These results suggest that Hdh-GR has central role in the stress physiology, immune response, gonadal development, and metamorphosis of Pacific abalone.
鲍鱼经常暴露于多种环境因素中，包括重金属毒性、热应激、HO 应激、饥饿、病毒和细菌感染，这些因素会诱导氧化应激。谷胱甘肽还原酶是抗氧化防御系统中的一种重要酶，可催化氧化型谷胱甘肽还原为还原型谷胱甘肽。本研究旨在鉴定和定位太平洋鲍（Hdh-GR）中的谷胱甘肽还原酶，并评估其在应激生理学、重金属毒性、免疫反应、性腺发育和变态中的潜在作用。Hdh-GR 的 mRNA 表达在热应激、饥饿、HO 应激和镉暴露毒性下上调。还在免疫 challenged 鲍中定量了诱导的 mRNA 表达。此外，在变态期间 Hdh-GR 的表达显着增加。Hdh-GR mRNA 表达与热应激的太平洋鲍中 ROS 产生呈负相关。这些结果表明 Hdh-GR 在太平洋鲍的应激生理学、免疫反应、性腺发育和变态中具有核心作用。
