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太平洋鲍鱼过氧化氢酶在应激生理、先天免疫、睾丸发育、变态及冷冻保存精子中的分子克隆与功能表征

Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone.

作者信息

Hossen Shaharior, Sukhan Zahid Parvez, Kim Soo Cheol, Hanif Md Abu, Kong Il-Keun, Kho Kang Hee

机构信息

Department of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University, 50 Daehak-ro, Yeosu 59626, Republic of Korea.

South Sea Fisheries Research Institute, National Institute of Fisheries Science, Yeosu 59780, Republic of Korea.

出版信息

Antioxidants (Basel). 2023 Jan 1;12(1):109. doi: 10.3390/antiox12010109.

DOI:10.3390/antiox12010109
PMID:36670971
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9854591/
Abstract

Catalase is a crucial enzyme of the antioxidant defense system responsible for the maintenance of cellular redox homeostasis. The aim of the present study was to evaluate the molecular regulation of catalase () in stress physiology, innate immunity, testicular development, metamorphosis, and cryopreserved sperm of Pacific abalone. gene was cloned from the digestive gland (DG) of Pacific abalone. The 2894 bp sequence of had an open reading frame of 1506 bp encoding 501 deduced amino acids. Fluorescence hybridization confirmed localization in the digestive tubules of the DG. was induced by different types of stress including thermal stress, HO induction, and starvation. Immune challenges with , lipopolysaccharides, and polyinosinic-polycytidylic acid sodium salt also upregulated mRNA expression and catalase activity. responded to cadmium induced-toxicity by increasing mRNA expression and catalase activity. Elevated seasonal temperature also altered mRNA expression. mRNA expression was relatively higher at the trochophore larvae stage of metamorphosis. Cryopreserved sperm showed significantly lower mRNA expression levels compared with fresh sperm. mRNA expression showed a relationship with the production of ROS. These results suggest that might play a role in stress physiology, innate immunity, testicular development, metamorphosis, and sperm cryo-tolerance of Pacific abalone.

摘要

过氧化氢酶是抗氧化防御系统中的一种关键酶，负责维持细胞氧化还原稳态。本研究的目的是评估太平洋鲍鱼过氧化氢酶（）在应激生理学、先天免疫、睾丸发育、变态以及冷冻保存精子方面的分子调控。从太平洋鲍鱼的消化腺（DG）中克隆了基因。的2894 bp序列有一个1506 bp的开放阅读框，编码501个推导氨基酸。荧光原位杂交证实了在DG消化小管中的定位。受到包括热应激、过氧化氢诱导和饥饿在内的不同类型应激的诱导。用、脂多糖和聚肌苷酸-聚胞苷酸钠盐进行免疫刺激也上调了mRNA表达和过氧化氢酶活性。通过增加mRNA表达和过氧化氢酶活性来应对镉诱导的毒性。季节性温度升高也改变了mRNA表达。在变态的担轮幼虫阶段，mRNA表达相对较高。与新鲜精子相比，冷冻保存的精子显示出明显较低的mRNA表达水平。mRNA表达与活性氧的产生有关。这些结果表明，可能在太平洋鲍鱼的应激生理学、先天免疫、睾丸发育、变态以及精子冷冻耐受性中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/0cc0ebe48a7a/antioxidants-12-00109-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/5609fccb694d/antioxidants-12-00109-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/a61e61afa08d/antioxidants-12-00109-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/6e28896c5670/antioxidants-12-00109-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/f28aba7d9a79/antioxidants-12-00109-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/826cd19b7519/antioxidants-12-00109-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/1489b57cfa5c/antioxidants-12-00109-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/7d61925eac1f/antioxidants-12-00109-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/4be9b3b5fe6f/antioxidants-12-00109-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/619b1663aa06/antioxidants-12-00109-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/0cc0ebe48a7a/antioxidants-12-00109-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/5609fccb694d/antioxidants-12-00109-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/a61e61afa08d/antioxidants-12-00109-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/6e28896c5670/antioxidants-12-00109-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/f28aba7d9a79/antioxidants-12-00109-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/826cd19b7519/antioxidants-12-00109-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/1489b57cfa5c/antioxidants-12-00109-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/7d61925eac1f/antioxidants-12-00109-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/4be9b3b5fe6f/antioxidants-12-00109-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/619b1663aa06/antioxidants-12-00109-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21cc/9854591/0cc0ebe48a7a/antioxidants-12-00109-g010.jpg

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