Aarons L J, Schary W L, Rowland M
J Pharm Pharmacol. 1979 May;31(5):322-30. doi: 10.1111/j.2042-7158.1979.tb13509.x.
The binding interactions between warfarin-salicylate and warfarin-phenylbutazone in the presence of 4 g percent bovine serum albumin at 37 degrees C were studied using equilibrium dialysis. Methods of representing and analysing drug binding interactions are discussed. Scatchard plots, double reciprocal plots and the like are shown to be of no use in representing drug displacement interactions since they display only one drug and they can be potentially misleading. It is argued that a preferable method of analysing drug displacement interaction data is in terms of a stepwise multiple equilibria model. The numerical problems associated with fitting this kind of model to the data are discussed. A three-dimensional representation of the binding surface is proposed as a superior means of visualizing drug displacement interactions.
在37摄氏度下，使用平衡透析法研究了华法林 - 水杨酸盐和华法林 - 苯基丁氮酮在4%牛血清白蛋白存在时的结合相互作用。讨论了表示和分析药物结合相互作用的方法。结果表明，Scatchard图、双倒数图等在表示药物置换相互作用时毫无用处，因为它们仅显示一种药物，可能会产生误导。有人认为，分析药物置换相互作用数据的一种更好方法是基于逐步多重平衡模型。讨论了将这类模型与数据拟合时相关的数值问题。提出了结合表面的三维表示法，作为可视化药物置换相互作用的一种更优手段。
