Erim F B, Kraak J C
Amsterdam Institute for Molecular Studies, Laboratory for Analytical Chemistry, University of Amsterdam, The Netherlands.
J Chromatogr B Biomed Sci Appl. 1998 Jun 12;710(1-2):205-10. doi: 10.1016/s0378-4347(98)00127-3.
The suitability of the vacancy affinity capillary electrophoretic method (VACE) for study of displacement of a target drug from a protein by simultaneously administered drugs was investigated. As test system, the displacement of warfarin from bovine serum albumin (BSA) by furosemide and phenylbutazone was selected. It appears that the displacement can be observed well from the shift of the actual mobility of warfarin when a displacer drug is added. Also, the competitive action of the displacer drugs (affinity for BSA) is clearly visible. The VACE method seems to be attractive for rapid assessment of information about the competitive properties of coadministered compounds.
研究了空位亲和毛细管电泳法（VACE）用于研究同时给药的药物从蛋白质中置换目标药物的适用性。作为测试系统，选择了速尿和保泰松从牛血清白蛋白（BSA）中置换华法林的情况。当加入置换药物时，似乎可以从华法林实际迁移率的变化中很好地观察到置换现象。此外，置换药物对BSA的竞争作用也清晰可见。VACE方法对于快速评估有关共同给药化合物竞争特性的信息似乎很有吸引力。
