Nagase Takashi, Ueno Koji, Mizoguchi Takahiro, Samura Makoto, Harada Takasuke, Suehiro Kotaro, Shirasawa Bungo, Morikage Noriyasu, Hamano Kimikazu
Department of Surgery and Clinical Sciences, Graduate School of Medicine, Yamaguchi University Ube, Yamaguchi, Japan.
Department of Medical Education, Yamaguchi University Graduate School of Medicine Ube, Japan.
Am J Transl Res. 2020 Jun 15;12(6):2652-2663. eCollection 2020.
BACKGROUND/AIMS: This study sought to confirm the difference of the wound-healing effect, cell survival, and immune response between autologous fibroblast sheets and allogeneic fibroblast sheets.
Regarding wound healing, autologous or allogeneic fibroblast sheets were transplanted onto a mouse cutaneous wound healing model and the wound contraction rate was evaluated. The luciferase-expressing fibroblast sheet was prepared and the survival of the cell sheet was evaluated by IVIS after autologous or allogeneic transplantation. Histological evaluation was performed at five and 14 days after transplantation.
Allogeneic fibroblast-sheet transplantation showed significant wound contraction at the early phase of wound healing, which was equivalent to that seen with the autologous fibroblast sheets. Luminescence of the autologous and allogeneic luciferase-expressing fibroblast sheets peaked on Day 5, and no luminescence was observed on Day 13. In the allogeneic fibroblast-sheet transplant group, a significant accumulation of immune cells was observed in the healed tissue but not in the early stage of wound healing.
The allogeneic fibroblast sheets showed comparable rates of cell survival and wound-healing effects to those of the autologous fibroblast sheets, despite the subsequent immunogenic response. This result supports the potential practical clinical application of scaffold-free allogeneic fibroblast sheets based on the paracrine effect.
背景/目的：本研究旨在确认自体成纤维细胞片和异体成纤维细胞片在伤口愈合效果、细胞存活及免疫反应方面的差异。
关于伤口愈合，将自体或异体成纤维细胞片移植到小鼠皮肤伤口愈合模型上，并评估伤口收缩率。制备表达荧光素酶的成纤维细胞片，在自体或异体移植后通过IVIS评估细胞片的存活情况。在移植后第5天和第14天进行组织学评估。
异体成纤维细胞片移植在伤口愈合早期显示出显著的伤口收缩，这与自体成纤维细胞片所见相当。自体和异体表达荧光素酶的成纤维细胞片的发光在第5天达到峰值，在第13天未观察到发光。在异体成纤维细胞片移植组中，在愈合组织中观察到免疫细胞显著聚集，但在伤口愈合早期未观察到。
尽管随后会发生免疫原性反应，但异体成纤维细胞片显示出与自体成纤维细胞片相当的细胞存活率和伤口愈合效果。这一结果支持了基于旁分泌效应的无支架异体成纤维细胞片在临床实际应用中的潜力。
