Evans Ben, Spell Evan, Bernstein Douglas
Biology, Ball State University, Muncie, Indiana, United States.
MicroPubl Biol. 2023 May 25;2023. doi: 10.17912/micropub.biology.000826. eCollection 2023.
is the most prevalent human fungal pathogen, and can be especially dangerous to immunocompromised individuals. One key aspect of virulence is morphological plasticity. can undergo a number of distinct morphological changes and these changes are controlled by complex transcriptional networks. The transcription factor Ume6 is an important member of these networks, playing an essential role mediating filamentation. , however encodes a second homolog, . is highly conserved in the CTG fungal clade, but the role of in biology is unknown. Here we truncate and delete . We find Ume7 is dispensable for growth and filamentation. We also find that deletion does not have major consequences on virulence or white opaque switching. Our results suggest that under standard laboratory conditions deletion of does not have large effects on phenotype leaving its role in biology undefined.
是最常见的人类真菌病原体,对免疫功能低下的个体尤其危险。毒力的一个关键方面是形态可塑性。可以经历许多不同的形态变化,这些变化由复杂的转录网络控制。转录因子Ume6是这些网络的重要成员,在介导丝状化中起重要作用。然而,编码第二个同系物。在CTG真菌进化枝中高度保守,但在生物学中的作用尚不清楚。在这里,我们截短并删除了。我们发现Ume7对于生长和丝状化是可有可无的。我们还发现缺失对毒力或白色不透明转换没有重大影响。我们的结果表明,在标准实验室条件下,缺失对表型没有太大影响,其在生物学中的作用尚不清楚。