叶酸功能化白蛋白/氧化石墨烯纳米复合材料同时递送姜黄素和 5-氟尿嘧啶进入人结肠癌细胞：一项研究。

Folic Acid-Functionalized Albumin/Graphene Oxide Nanocomposite to Simultaneously Deliver Curcumin and 5-Fluorouracil into Human Colorectal Cancer Cells: An Study.

机构信息

Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.

Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.

出版信息

Biomed Res Int. 2023 May 31;2023:8334102. doi: 10.1155/2023/8334102. eCollection 2023.

DOI:10.1155/2023/8334102

PMID:37304465

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10256446/

Abstract

BACKGROUND

Nowadays, due to various inherent properties, graphene-based nanoparticles are widely used in drug delivery research. On the other hand, folate receptors are highly expressed on the surface of human tumor cells. In this work, to enhance the 5-fluorouracil (5FU) and curcumin (Cur) effects on colon cancer, we constructed a folic acid- (FA-) modified codelivery carrier based on graphene nanoparticles (GO-Alb-Cur-FA-5FU).

MATERIALS AND METHODS

The HUVEC and HT-29 were selected for evaluating the antitumor effect of the prepared nanocarriers. The structure of nanocarriers was characterized by FTIR spectroscopy, X-ray diffraction analysis, TEM microscopy, and a DLS analyzer. The efficiency of the prepared carrier was evaluated by fluorescence microscopy using Annexin V and the PI kit. The cytotoxicity of the carrier's component individually and the efficacy of the drug carrier GO-Alb-Cur-FA-5FU were assessed by MTT.

RESULTS

The results of the pharmacological tests indicated that the new nanoparticles cause increased apparent toxicity in HT-29 cells. The apoptosis rate of the HT-29 and HUVEC cells treated with IC50 values of GO-Alb-Cur-FA-5FU for 48 h was higher than the cells treated with IC50 values of 5FU and Cur individually, which indicated the greater inhibitory efficacy of GO-Alb-Cur-FA-5FU than free drugs.

CONCLUSION

The designed GO-Alb-CUR-FA-5FU delivery system can be applied for targeting colon cancer cells and can be severe as a potential candidate for future drug development.

摘要

背景

如今，由于各种固有特性，基于石墨烯的纳米粒子在药物输送研究中得到了广泛应用。另一方面，叶酸受体在人类肿瘤细胞表面高度表达。在这项工作中，为了增强 5-氟尿嘧啶（5FU）和姜黄素（Cur）对结肠癌的作用，我们构建了基于石墨烯纳米粒子（GO-Alb-Cur-FA-5FU）的叶酸（FA）修饰的共递药载体。

材料与方法

选择 HUVEC 和 HT-29 来评估所制备的纳米载体的抗肿瘤效果。通过傅里叶变换红外光谱（FTIR）、X 射线衍射分析（XRD）、TEM 显微镜和 DLS 分析仪对纳米载体的结构进行了表征。通过 Annexin V 和 PI 试剂盒的荧光显微镜评估了制备载体的效率。通过 MTT 评估了载体成分的单独细胞毒性和药物载体 GO-Alb-Cur-FA-5FU 的疗效。