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含姜黄素的精氨酸、甘氨酸、天冬氨酸肽修饰纳米脂质体在大鼠体内的生物分布评价

Evaluation of the Biodistribution of Arginine, glycine, aspartic acid peptide-modified Nanoliposomes Containing Curcumin in Rats.

作者信息

Salehi Najafabadi Parisa, Delaviz Hamdollah, Asfaram Arash, Jafari Barmak Mehrzad, Ghanbari Amir, Alipour Mohsen, Rad Parastou, Bardania Hassan

机构信息

Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.

Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.

出版信息

Iran J Biotechnol. 2022 Apr 1;20(2):e2990. doi: 10.30498/ijb.2021.272168.2990. eCollection 2022 Apr.

DOI:10.30498/ijb.2021.272168.2990
PMID:36337060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9583817/
Abstract

BACKGROUND

Liposomes, as a biological membrane, is successfully used for drug delivery, reduces toxicity in normal cells and improves bio-accessibility of the drug to the target cells. Curcumin, as a bioactive substance with pleiotropic biological activities, is an anti-inflammatory compound and has several anticancer effects in different cancers such as pancreatic and breast cancer.

OBJECTIVES

This study was conducted to determine the bio-distribution of arginine-glycine-aspartic acid (RGD)-modified nanoliposomes containing curcumin in different tissues of rats.

MATERIALS AND METHODS

The amount of curcumin in each tissue was examined by HPLC analysis. The distribution of liposomal Hoechst in the rats was evaluated by using fluorescence spectrophotometry, live animal imaging analyses and histological methods.

RESULTS

HPLC analysis showed the mean of curcumin in the blood significantly increased in the liposomal curcumin modified with RGD compared to free curcumin. These results were confirmed by fluorescence measurement for RGD modified liposome containing Hoechst dye. There was negligible fluorescent intensity in the blood rats, which received Hoechst alone. Live animal imaging analysis showed the presence of fluorescent color in heart tissue for all groups. It was also detected in kidney tissue for liposomal Hoechst modified with RGD group.

CONCLUSIONS

The present study demonstrated that RGD-modified nano-liposomes can significantly improve drug retention time in the blood of rats.

摘要

背景

脂质体作为一种生物膜，已成功用于药物递送，可降低对正常细胞的毒性并提高药物对靶细胞的生物可及性。姜黄素作为一种具有多种生物活性的生物活性物质，是一种抗炎化合物，在胰腺癌和乳腺癌等不同癌症中具有多种抗癌作用。

目的

本研究旨在确定含姜黄素的精氨酸-甘氨酸-天冬氨酸（RGD）修饰纳米脂质体在大鼠不同组织中的生物分布。

材料与方法

通过高效液相色谱（HPLC）分析检测各组织中姜黄素的含量。采用荧光分光光度法、活体动物成像分析和组织学方法评估脂质体荷因在大鼠体内的分布。

结果

HPLC分析表明，与游离姜黄素相比，RGD修饰的脂质体姜黄素在血液中的姜黄素平均值显著增加。含Hoechst染料的RGD修饰脂质体的荧光测量结果证实了这些结果。单独接受Hoechst的大鼠血液中荧光强度可忽略不计。活体动物成像分析显示所有组的心脏组织中均存在荧光颜色。在RGD修饰的脂质体荷因组的肾脏组织中也检测到了荧光。

结论

本研究表明，RGD修饰的纳米脂质体可显著提高大鼠血液中的药物保留时间。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/6ab5e219c528/IJB-20-e2990-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/abcb64b9c3d2/IJB-20-e2990-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/93873f2ddee8/IJB-20-e2990-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/fc6bc145bb1c/IJB-20-e2990-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/8f80b112ba35/IJB-20-e2990-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/36cf1eb774ea/IJB-20-e2990-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/6ab5e219c528/IJB-20-e2990-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/abcb64b9c3d2/IJB-20-e2990-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/93873f2ddee8/IJB-20-e2990-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/fc6bc145bb1c/IJB-20-e2990-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/8f80b112ba35/IJB-20-e2990-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/36cf1eb774ea/IJB-20-e2990-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c283/9583817/6ab5e219c528/IJB-20-e2990-g006.jpg

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