Institute for Cell Analysis, Shenzhen Bay Laboratory, Shenzhen, Guangdong, China.
Adv Biochem Eng Biotechnol. 2023;186:51-76. doi: 10.1007/10_2023_220.
Biosynthesis involving multi-enzymatic reactions is usually an efficient and economic method to produce plentiful important molecules. To increase the product yield in biosynthesis, the involved enzymes can be immobilized to carriers for enhancing enzyme stability, increasing synthesis efficiency and improving enzyme recyclability. Hydrogels with three-dimensional porous structures and versatile functional groups are promising carriers for enzyme immobilization. Herein, we review the recent advances of the hydrogel-based multi-enzymatic system for biosynthesis. First, we introduce the strategies of enzyme immobilization in hydrogel, including the pros and cons of the strategies. Then we overview the recent applications of the multi-enzymatic system for biosynthesis, including cell-free protein synthesis (CFPS) and non-protein synthesis, especially high value-added molecules. In the last section, we discuss the future perspective of the hydrogel-based multi-enzymatic system for biosynthesis.
涉及多酶反应的生物合成通常是生产大量重要分子的有效且经济的方法。为了提高生物合成中的产物产量，可以将相关酶固定在载体上，以增强酶稳定性、提高合成效率和改善酶可回收性。具有三维多孔结构和多功能基团的水凝胶是酶固定化的有前途的载体。本文综述了基于水凝胶的多酶生物合成系统的最新进展。首先，我们介绍了酶在水凝胶中的固定化策略，包括策略的优缺点。然后我们综述了多酶系统在生物合成中的最新应用，包括无细胞蛋白合成（CFPS）和非蛋白合成，特别是高附加值分子。在最后一节中，我们讨论了基于水凝胶的多酶生物合成系统的未来展望。
