Institut Pasteur, Université de Paris, Unité d'Architecture et de Dynamique des Macromolécules Biologiques, Paris, France.
Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Donostia-San Sebastián, Spain.
Adv Biochem Eng Biotechnol. 2023;185:91-127. doi: 10.1007/10_2023_224.
The use of cell-free production systems in droplet microfluidic devices has gained significant interest during the last decade. Encapsulating DNA replication, RNA transcription, and protein expression systems in water-in-oil drops allows for the interrogation of unique molecules and high-throughput screening of libraries of industrial and biomedical interest. Furthermore, the use of such systems in closed compartments enables the evaluation of various properties of novel synthetic or minimal cells. In this chapter, we review the latest advances in the usage of the cell-free macromolecule production toolbox in droplets, with a special emphasis on new on-chip technologies for the amplification, transcription, expression, screening, and directed evolution of biomolecules.
在过去十年中，无细胞生产系统在液滴微流控装置中的应用引起了极大的关注。将 DNA 复制、RNA 转录和蛋白质表达系统包封在油包水液滴中，可以研究独特的分子，并高通量筛选具有工业和生物医学意义的文库。此外，此类系统在封闭隔室中的使用可以评估新型合成或最小细胞的各种特性。在本章中，我们回顾了无细胞大分子生产工具箱在液滴中的最新应用进展，特别强调了用于生物分子的扩增、转录、表达、筛选和定向进化的新型片上技术。
