Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China; Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, China.
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Exp Cell Res. 2023 Aug 15;429(2):113686. doi: 10.1016/j.yexcr.2023.113686. Epub 2023 Jun 10.
Prostate cancer (PCa) is one of the most deadly and metastatic cancers of the urinary tract. Latest studies have confirmed that long non-coding RNAs (lncRNAs) play a crucial role in a variety of cancers. Some of these lncRNAs code for small nucleolar RNAs (snoRNAs), called small nucleolar RNA host genes (SNHGs), which exert some value in predicting the prognosis of certain cancer patients, but little is known regarding the function of SNHGs within the PCa.
To explore the expression distribution and differential analysis of SNHGs in different tumors using RNA-seq and survival data from TCGA and GTEx, and to assess the potential impacts of the lncRNA SNHG25 on human PCa. To validate the expression of SNHG25 using experimental data and to investigate in detail its particular molecular biological function on PCa both in vivo and in vitro.
LncRNA SNHG25 expression was analyzed by bioinformatic prediction and qPCR. CCK-8, EdU, transwell, wound healing, and western blotting assays were conducted to investigate the main role of lncRNA SNHG25 in PCa. Xenograft tumour growth model in nude mice was surveyed by in vivo imaging and Ki-67 staining. AKT pathway activator (SC79) was used to verify the interaction among SNHG25 and PI3K/AKT signaling pathway.
Bioinformatics analysis and experimental research illuminated that the expression of lncRNA SNHG25 was observably up-regulated in PCa tissues and cells. Moreover, SNHG25 knockdown restrained PCa cell proliferation, invasion and migration, while promoting apoptosis. Xenografts model confirmed that the si-SNHG25 group had a significant inhibitory effect on PCa tumour growth in vivo. Additionally, a series of gain-of-function analyses suggested that SNHG25 could activate the PI3K/AKT pathway to accelerate PCa progression.
These in vitro and in vivo findings demonstrate that SNHG25 is highly expressed in PCa and facilitates PCa development through regulation of PI3K/AKT signaling pathway. SNHG25 acts as an oncogene to predict tumour malignancy and survival in PCa patients and may therefore become a promising potential molecular target for early detection and therapy of lethal PCa.
前列腺癌（PCa）是泌尿系统中最致命和转移性的癌症之一。最新研究证实，长链非编码 RNA（lncRNA）在多种癌症中发挥着关键作用。其中一些 lncRNA 编码小核仁 RNA（snoRNA），称为小核仁 RNA 宿主基因（SNHG），它们在预测某些癌症患者的预后方面具有一定的价值，但关于 SNHG 在 PCa 中的功能知之甚少。
使用 RNA-seq 技术和 TCGA 和 GTEx 的生存数据，探讨 SNHG 在不同肿瘤中的表达分布和差异分析，并评估 lncRNA SNHG25 对人 PCa 的潜在影响。使用实验数据验证 SNHG25 的表达，并详细研究其在体内和体外对 PCa 的特定分子生物学功能。
通过生物信息学预测和 qPCR 分析 lncRNA SNHG25 的表达。通过 CCK-8、EdU、transwell、划痕愈合和 Western blot 实验研究 lncRNA SNHG25 在 PCa 中的主要作用。通过体内成像和 Ki-67 染色研究裸鼠移植瘤生长模型。使用 AKT 通路激活剂（SC79）验证 SNHG25 与 PI3K/AKT 信号通路之间的相互作用。
生物信息学分析和实验研究表明，lncRNA SNHG25 在 PCa 组织和细胞中表达明显上调。此外，SNHG25 下调抑制了 PCa 细胞的增殖、侵袭和迁移，同时促进了凋亡。移植瘤模型证实，si-SNHG25 组在体内对 PCa 肿瘤生长具有显著的抑制作用。此外，一系列功能获得分析表明，SNHG25 可以激活 PI3K/AKT 通路加速 PCa 进展。
这些体内外研究结果表明，SNHG25 在 PCa 中高表达，并通过调节 PI3K/AKT 信号通路促进 PCa 的发展。SNHG25 作为一种癌基因，可预测 PCa 患者的肿瘤恶性程度和生存情况，因此可能成为早期检测和治疗致命性 PCa 的有前途的潜在分子靶标。
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