蛋白质笼组装的时间分辨小角 X 射线散射。
Time-Resolved Small-Angle X-Ray Scattering of Protein Cage Assembly.
机构信息
Department of Bioinformatics, Soka University, Hachioji, Tokyo, Japan.
RIKEN SPring-8 Center, Sayo, Hyogo, Japan.
出版信息
Methods Mol Biol. 2023;2671:211-218. doi: 10.1007/978-1-0716-3222-2_12.
PMID:37308647
Abstract
Recent improvements in X-ray detectors and synchrotron light sources have made it possible to measure time-resolved small-angle X-ray scattering (TR-SAXS) at millisecond time resolution. As an example, in this chapter we describe the beamline setup, experimental scheme, and the points that should be noted in stopped-flow TR-SAXS experiments for investigating the ferritin assembly reaction.
摘要
近年来，X 射线探测器和同步加速器光源的改进使得在毫秒时间分辨率下测量时间分辨小角 X 射线散射（TR-SAXS）成为可能。作为一个例子，在这一章中，我们描述了用于研究铁蛋白组装反应的流停 TR-SAXS 实验中的光束线设置、实验方案以及应注意的要点。
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